Proteomics

Dataset Information

0

A sub-compartment proteome for murine sensory neurons


ABSTRACT: We applied TurboID-based proximity labelling restricted to Advillin-expressing neurons to systematically profile the proteomes of distinct neuronal subcompartments, using parallel accumulation–serial fragmentation combined with data-independent acquisition (diaPASEF) mass spectrometry. Neuronal compartments include somata (L3–L5 dorsal root ganglia, DRG), presynaptic terminals (dorsal lumbar spinal cord; dLSC), axons (sciatic nerve; SCN), and peripheral terminals (paw skin), providing the first proteomic atlas derived of/enriched in lumbar sensory neurons. Our findings provide novel insights into the compartment-specific protein distributions that underlie key aspects of sensory neuron function. To highlight to effectiveness of this approach. we also explored the neuronal proteome on DRG explants exposed to Oxaliplatin (or Vehicle) to extract neuron-specific changes upon chemotherapy exposure. In both cases, the corresponding, wildtype (whole tissue) proteomes were also profiled with diaPASEF. Together, these data serve as a valuable resource for future studies on neuronal specialization and pain-related proteomic alterations in the PNS.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Nerve, Spinal Ganglion, Spinal Cord, Skin

SUBMITTER: Allison Barry  

LAB HEAD: Manuela Schmidt

PROVIDER: PXD062198 | Pride | 2026-04-14

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
TurboID-compartments-matrix.csv Csv
TurboID-compartments-quant.zip Other
TurboID-compartments-search.zip Other
TurboID-compartments.zip Other
TurboID-explants-matrix.csv Csv
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