Dataset Information

ZooMS and Shotgun Proteomics for Species Identification of the Nile Softshell Turtle (Trionyx triunguis)

ABSTRACT: We applied nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) in a single Trionyx triunguis reference specimen to identify diagnostic COl1 peptide markers. Our analysis revealed four COL1 biomarkers that differentiate T. triunguis from all sea turtle species, as well as seven additional turtle species with COL1 sequences available on NCBI: Chelonoidis abingdonii, Chrysemys picta bellii, Gopherus evgoodei, Mauremys reevesii, Pelodiscus sinensis, Terrapene carolina triunguis, and Trachemys scripta elegans. These findings enhance biomolecular identification methods for freshwater and marine turtle remains in archaeological and ecological contexts (especially on the Levant).

INSTRUMENT(S):

ORGANISM(S): Trionyx Triunguis

TISSUE(S): Bone

SUBMITTER: Willemien de Kock

LAB HEAD: Matthew Collins

PROVIDER: PXD062259 | Pride | 2026-02-16

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20200908_EXPL8_nLC4_MEM_collab_77min_DDA_Ancient_bone_T_triunguis_M10_01.raw	Raw
	MALDIs.zip	Other
	checksum.txt	Txt
	txt.zip	Other

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Publications

Stable isotope evidence of anthropocene disruption in African softshell turtle foraging.

de Kock Willemien W van der Meer Marcel T J MTJ van Bommel Ronald R Taurozzi Alberto J AJ Von Tersch Matthew M Allentoft Morten E ME Mackie Meaghan M Ramsøe Max M Collins Matthew M Alexander Michelle M Palsbøll Per J PJ Çakırlar Canan C Turkozan Oguz O

PloS one 20260211 2

We examined the dietary habits of contemporary and Middle to Late Holocene (ancient) populations of African softshell turtles (Trionyx triunguis) from the northern Levant using stable isotope analysis (δ13C, δ15N, and δ34S) and ZooMS biomarker identification. Our study presents the first application of ZooMS to this taxon, facilitating species-level identification. Stable isotope values point to potential variation in T. triunguis diets, possibly reflecting changing ecosystem conditions. Modern ...[more]

PMID: 41671206

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