CAPTURE2.0 pull-down of SINE-MIR associated proteins in mouse embryonic stem cells

ABSTRACT: In mouse embryonic stem cells, the regulators of transposable elements are not well known. Here, we studied the proteins regulating mammalian-wide interspersed repeat (MIR), a short-interspersed nuclear elements (SINE) family using the CAPTURE2.0 system. We generated cell lines expressing biotinylated dCas9 protein and MIR-targeting sgRNAs, performed biotin pulldown to isolate MIR-associated proteins, and analyzed them using mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Yee Jiun Kok  

LAB HEAD: Xuezhi Bi

PROVIDER: PXD062330 | Pride | 2025-04-01

REPOSITORIES: Pride