Dataset Information

A Pseudomonas aeruginosa biliverdin IX-dependent transcriptional regulator (BdrB) integrates extracellular heme metabolism with Type IV pili-dependent motilities

ABSTRACT: Pseudomonas aeruginosa displays remarkable adaptability in chronic infections by exploiting heme as its primary iron source at the expense of siderophore uptake. Previous studies have identified a pivotal role for the heme metabolites BVIX β/δ in the regulation of Type IV pili-dependent motility and biofilm formation. Thus, the heme metabolites integrate the switch in iron source with lifestyle adaptations required for chronic infection. The mechanism by which the BVIX metabolites directly or indirectly regulate such lifestyle adaptations remains elusive. In this study, through a combination of thermal proteome profiling (TPP) followed by quantitative mass spectrometry (MS), we identified a BVIX-binding protein PA5271 encoded in a three-gene operon (pa5269-pa-5270-pa5271). PA5271 is annotated as a helix-turn-helix DNA-binding protein and purifies as a dimer that binds BVIX but not BVIX or BVIX. Deletion of the gene renamed bdrB (biliverdin-dependent regulator responsive to BVIX) displayed a marked reduction in Type IV pili (TFP) associated motilities including twitching.

INSTRUMENT(S):

ORGANISM(S): Pseudomonas Sp. Ar40

SUBMITTER: Mehari Weldemariam

LAB HEAD: Maureen A. Kane

PROVIDER: PXD062383 | Pride | 2026-06-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	WH_PAO1_TPP_Ctrl_47C_1ug_3-5ul_1_redo_12-15-2016.mgf	Mgf
	WH_PAO1_TPP_Ctrl_47C_1ug_3-5ul_2_12-12-2016.mgf	Mgf
	WH_PAO1_TPP_Ctrl_47C_1ug_3-5ul_3_12-12-2016.mgf	Mgf
	WH_PAO1_TPP_Ctrl_50C_1ug_3-5ul_1_12-12-2016.-1326212816.mznld	Other
	WH_PAO1_TPP_Ctrl_50C_1ug_3-5ul_1_redo_12-15-2016.mgf	Mgf

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Publications

pmultiqc: An Open-Source, Lightweight, and Metadata-Oriented QC Reporting Library for MS Proteomics.

Yue Qi-Xuan QX Dai Chengxin C Kamatchinathan Selvakumar S Bandla Chakradhar C Webel Henry H Larrea Asier A Bittremieux Wout W Uszkoreit Julian J Müller Tom David TD Xiao Jinqiu J Cox Juergen J Yu Fengchao F Ewels Philip P Demichev Vadim V Kohlbacher Oliver O Sachsenberg Timo T Bielow Chris C Bai Mingze M Perez-Riverol Yasset Y

Molecular & cellular proteomics : MCP 20260217 6

The increasing scale and complexity of proteomics data demand robust, scalable, and interpretable quality control (QC) frameworks to ensure data reliability and reproducibility. Here, we present pmultiqc, an open-source Python package that standardizes and generates web-based QC reports across multiple proteomics data analysis platforms. Built on top of the widely adopted MultiQC framework, pmultiqc offers specialized modules tailored to mass spectrometry workflows, with full initial support for ...[more]

PMID: 41713790

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Project description:Background: Haemophilus influenzae has an absolute aerobic growth requirement for heme and has developed multiple complex mechanisms to obtain this essential nutrient. Although an understanding of the heme acquisition mechanisms of H. influenzae is emerging, significant gaps remain to be elucidated. In a previous study we utilized H. influenzae strain Rd KW20 to demonstrate the utility of transcriptional profiling in defining the genes exhibiting altered transcription in response to environmental iron and heme levels. The current study expands upon those initial observations by determining the iron/heme regulons of two additional H. influenzae clinical isolates, i.e. the type b isolate 10810 and the nontypeable isolate R2866, to characterize the core iron/heme regulon of the species. Results: A microarray chip was designed to incorporate probes for all of the genes of H. influenzae isolates 10810 and R2866, and microarray studies were performed to compare gene expression under iron/heme-replete and iron/heme-restricted conditions for each isolate. Of 1820 ORFs on the array corresponding to R2866 genes, 363 were significantly differentially expressed. Of these 363 genes, 233 were maximally transcribed under iron/heme-replete conditions and 130 were preferentially transcribed in iron/heme-restricted conditions. Of the 1883 ORFs representing genes of strain10810, 351 were significantly differentially transcribed, 150 of these were preferentially transcribed in iron/heme-replete conditions and 201 were preferentially transcribed in iron/heme-restricted conditions. Comparison of the data sets indicated that 163 genes were similarly regulated in both isolates and that 74 of these also exhibited similar patterns of regulation in strain Rd KW20. Conclusion: This study provides evidence for a core of H. influenzae genes that are regulated by the availability of iron and/or heme in the growth environment. Elucidation of this core regulon provides targets for investigation of genes with an unrecognized role in iron and heme homeostasis, as well as other potential virulence determinants. In addition, the set of core genes potentially provides targets for therapeutic and vaccine designs since these products of these genes are likely to be preferentially expressed during growth in iron/heme restricted sites of the human body. Keywords: Transcription analyses Three replicate cultures were grown in iron and heme depleted media for 90 minutes and samples taken for expression analyses. Fe and heme were supplemented to the remaining cultures and the cultures allowed to incubate an additional 20 minutes. Microarray analyses were used to determine changes in gene expression resulting from transfer from FeHm-deplete to FeHm-replete conditions.