Immunoprecipitation-Mass Spectrometry (IP-MS) of SRPK1 in endothelial cells

ABSTRACT: To identify the E3 ubiquitin ligase responsible for the polyubiquitination of SRPK1, we performed immunoprecipitation (IP) using an anti-SRPK1 antibody or IgG control as well as endothelial lysates treated with PA and MG-132, followed by mass spectrometry analysis

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Primary Cell, Endothelial Cell

SUBMITTER: Xiao-Hui Tan  

LAB HEAD: Rui-Li GUAN

PROVIDER: PXD062417 | Pride | 2026-05-30

REPOSITORIES: Pride