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Identification of NapM protein interactors in Mycobacterium smegmatis

ABSTRACT: Nucleoid-associated proteins (NAPs) are key factors in maintaining chromosomal DNA integrity, gene regulation, DNA replication, and DNA repair. A recently identified mycobacterial NAP, NapMin (NapM) from Mycobacterium smegmatis, was a subject of the study. NapM modulates the expression of about one-third of M. smegmatis genes. Among these genes, those involved in cell envelope biosynthesis and cell division comprise a particular group specific to NapM. A bottom-up proteomics approach was utilized to define NapM interactors on protein level. For this purpose, a NapM pull-down was first performed from M. smegmatis cell lysate on antiFlagTag resin. Proteins associated with the resin were then identified via mass spectrometry. A WT cell lysate sample was parallelly processed in the exact same manner as background control.

INSTRUMENT(S):

ORGANISM(S): Mycobacterium Smegmatis

TISSUE(S): Cell Suspension Culture

SUBMITTER: Michał Tracz

LAB HEAD: dr Michał Tracz

PROVIDER: PXD062473 | Pride | 2026-05-25

REPOSITORIES: Pride

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			Action	DRS
	250307_ZMM_EKR_N1.raw.zip	Raw
	250307_ZMM_EKR_N2.raw.zip	Raw
	250307_ZMM_KMI_N1.raw.zip	Raw
	250307_ZMM_KMI_N2.raw.zip	Raw
	250319_ZMM_KMI_WT1.raw.zip	Raw

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