Dataset Information

Production of multifunctional hydrolysates from Lupinus mutabilis protein using a Micrococcus sp. PC7 protease

ABSTRACT: The growing demand for functional foods has driven the search for bioactive com-pounds derived from plant proteins. Lupinus mutabilis “Tarwi,” a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bi-oactive peptides (BPs). In this study, the functionality of the proteins contained in the albumin fraction (AF) isolated by tangential ultrafiltration (TFF) was investigated by using the OmicsBox software. The identified proteins were functionally classified into three groups: cellular components (35.57%), molecular function (33.45%), and biological process (30.97%). The isolated AF was hydrolyzed with the native protease PC7 (HAP), optimizing the E/S ratio and time parameters. Additionally, sequential hydrolysis of the PC7 protease and alcalase (HAPA) was performed. In vitro multifunctionality assays, HAP and HAPA demonstrated the ability to scavenge radicals (ABTS and ORAC), in-hibit angiotensin-converting enzyme (ACE)-I, and dipeptidyl peptidase IV (DPP-IV). The findings of this study highlight the potential of L. mutabilis albumin hydrolysate as a multifunctional ingredient for functional foods aimed at managing chronic conditions associated with oxidative stress, hypertension, and/or metabolic disorders.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Lupinus Mutabilis Phaseoleae Lupinus Angustifolius Lupinus Albus

SUBMITTER: Samuel Paterson Moreno

LAB HEAD: Blanca Hernández Ledesma

PROVIDER: PXD062562 | Pride | 2025-06-02

REPOSITORIES: Pride

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Production of Multifunctional Hydrolysates from the Lupinus mutabilis Protein Using a Micrococcus sp. PC7 Protease.

Llontop-Bernabé Keyla Sofía KS Intiquilla Arturo A Ramirez-Veliz Carlos C Santos Marco M Jiménez-Aliaga Karim K Zavaleta Amparo Iris AI Paterson Samuel S Hernández-Ledesma Blanca B

Biotech (Basel (Switzerland)) 20250427 2

The growing demand for functional foods has driven the search for bioactive compounds derived from plant proteins. Lupinus mutabilis "Tarwi", a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bioactive peptides (BPs). In this study, the functionality of the proteins contained in the albumin fraction (AF) isolated by tangential ultrafiltration (TFF) was investigated by using the OmicsBox software. The identified proteins were functi ...[more]

PMID: 40407487

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Project description:Single-walled carbon nanotubes (SWCNTs) are being explored and used for a wide range of applications in industrial and medical sectors, and the increasing exposure of SWCNTs necessitate the studies of their potential environmental and health effects. Considerable efforts have been made to improve the dispersion of SWCNTs by chemical modifications. The present study was designed to determine if the acid functionalization of SWCNTs enhanced the toxicity and more efforts were made to understand the molecular mechanisms. RAW264.7 cells were exposed to 0-100μg/mL of SWCNTs and AF-SWCNTs for 24 hours and cell proliferation, viability, and gene expression profiles were assessed and compared. Results showed that the magnitude of AF-SWCNT-induced cell proliferation inhibition was higher than that of SWCNTs. 10μg/mL of AF-SWCNTs was determined as non-toxic to cells by viability analysis. AF-SWCNTs could enter and aggregate in cell cytoplasm and nuclear areas. Microarray, real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assays (ELISA) demonstrated that AF-SWCNTs (1, 10, and 50μg/mL) altered gene and protein expression patterns. The most differentially expressed genes were related to pro-inflammatory cytokines and chemokines (such as CCL-3 and CCL-4), apoptosis (e.g., Caspase-1, -4, and Dffa), protective response (such as Hmox-1and GSTM3), and cell surface molecules such as ICAM-1, Itgb2, indicating a possible involvement of SWCNT in inflammation, anti-apoptotic and DNA fragmentation, carcinogenesis and biased T cell polarization. Furthermore, a substantial amount of down-regulated genes were found related to ribosomal assembly and mitochondrial respiration chain, implicating a possible mechanism of SWCNT-induced oxidative stress and gene expression inhibition. RAW264.7 gene expression after exposure to 1-50ug/ml AF-SWCNT-24 hours. 0ug/ml of AF-SWCNT was used as control sample. The data were obtained from three biological replicates.

Project description:ABSTRACT Background: Primary human distal lung/parenchymal fibroblasts (DLF) exhibit a different phenotype from airway fibroblasts (AF), including the expression of high levels of alpha-SMA. The scope of the differences and the mechanisms driving them are unknown. We hypothesized that the distinct fibroblast characteristics based on lung region predicted a broad range of genomic differences which contribute to distinctly different functional pathway activation in AF and DLF. Methods and Findings: To investigate whether comprehensive gene expression patterns vary between AF and DLF, we compared global gene expression profiles of 3 matched pairs of primary human fibroblasts isolated from proximal and distal lung. 194 transcripts were upregulated in AF, and 290 transcripts upregulated in DLF. Quantitative real-time PCR (qRT-PCR) from both asthmatic and normal subjects confirmed the validity of microarray data (n = 8). Further analysis identified distinct pathway activation patterns, including the identification of the TGF-β receptor/SMAD3 signaling pathway as critical to the phenotypic differences in AF and DLF. The functional impact of these molecular differences on AF and DLF was then analyzed using Western blot, qRT-PCR, ELISA and gene knock-down approaches. TGF-β receptor/SMAD2-3 pathway analysis confirmed the contribution of higher TGF-β1 expression and accompanying SMAD3 and JNK activation to the increased alpha-SMA level seen in DLF. There are two limitations associated with our work. First, the microarray sample size was small. Second, for ethical reasons, a large majority of our data were from asthmatic subjects, only one pair of airway and distal lung tissues from a subject without knowing preexisting lung disease matched for age and smoking status was available. In this single normal subject, the same pattern of differences existed, supporting the concept that these genomic differences are regional, rather than disease-related. Conclusions: These findings demonstrated marked molecular and functional differences for these two lung regional fibroblast populations. These results suggest that airway and parenchymal fibroblasts differ in their responses to injury, repair, and remodeling in the lungs. There are likely profound implications of these observations for understanding mechanisms of development of fibrotic lung diseases as well as approaching therapy. In order to identify the underlying molecular differences between airway- and distal lung fibroblasts, microarray analysis was performed on 3 different matched pairs of fibroblasts.

			Action	DRS
	180624_Arturo_T1.mgf	Mgf
	180624_Arturo_T1.raw.mzxml	Mzxml
	180624_Arturo_T1.xml	Xml
	AF.mgf	Mgf
	AF.raw	Raw

Dataset Information

Production of multifunctional hydrolysates from Lupinus mutabilis protein using a Micrococcus sp. PC7 protease

Dataset's files

Publications

Production of Multifunctional Hydrolysates from the <i>Lupinus mutabilis</i> Protein Using a <i>Micrococcus</i> sp. PC7 Protease.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Production of multifunctional hydrolysates from Lupinus mutabilis protein using a Micrococcus sp. PC7 protease

Dataset's files

Publications

Production of Multifunctional Hydrolysates from the &lt;i&gt;Lupinus mutabilis&lt;/i&gt; Protein Using a &lt;i&gt;Micrococcus&lt;/i&gt; sp. PC7 Protease.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Production of Multifunctional Hydrolysates from the <i>Lupinus mutabilis</i> Protein Using a <i>Micrococcus</i> sp. PC7 Protease.