Dataset Information

Thermal Proteome Analysis of nutrient stress in Cupriavidus necator expression dataset

ABSTRACT: The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources or of autotrophic growth using CO2 fixation with H2 as an energy source. Under stress conditions, it is capable of producing biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total biomass. This metabolic versatility means that C. necator is under intense study for sustainable biotechnology processes; however a relative lack of understanding of the overall regulatory architecture has limited its application. The major mechanisms by which proteins can respond to shifting cellular demands are protein expression change and/or allosteric regulation. Here we use two powerful proteomics methods to investigate these responses in C. necator cells grown on balanced and low nitrogen (PHA-inducing) media. Using quantitative proteomics and protein stability analysis (which can report on conformation change), we find that proteins across different pathways respond through one or both of these regulatory modes, including coordinated adaption to nutrient stress by the PHA pathway, the Calvin cycle, and ribosomal proteins.

INSTRUMENT(S):

ORGANISM(S): Cupriavidus Necator (strain Atcc 17699 / H16 / Dsm 428 / Stanier 337) (ralstonia Eutropha) Bacteria

SUBMITTER: Eugene Dillon

LAB HEAD: Gerard Cagney

PROVIDER: PXD062592 | Pride | 2025-11-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	230623kw_KateTMT_F1_20230626154247.raw	Raw
	230623kw_KateTMT_F2_20230626103721.raw	Raw
	230623kw_KateTMT_F3_20230626204606.raw	Raw
	230623kw_KateTMT_N1.raw	Raw
	230623kw_KateTMT_N2.raw	Raw

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Publications

Response to Nutrient Stress in the Industrial Model Bacterium Cupriavidus necator: A Thermal Proteome Profiling (TPP) Investigation.

McKeever Kate K Tham Jia-Lynn JL Bruch Manuel M Narancic Tania T Connor Kevin O' KO Upadhya Swathi Ramachandra SR Ryan Colm C Dillon Eugene T ET Wynne Kieran K Cagney Gerard G

Proteomics 20250916 20

The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources, or of autotrophic growth using CO<sub>2</sub> fixation with H<sub>2</sub> as an energy source. Under stress conditions, it produces biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total biomass. This metabolic versatility means that C. necator is under intense study for sustainable biotechnology processes; however, a ...[more]

PMID: 40955630

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