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Changes in histone abundance and post-translational modifications in Rana [Lithobates] catesbeiana tissues during temperature-mediated thyroid hormone-induced metamorphosis

ABSTRACT: Amphibian metamorphosis involves thyroid hormone (TH)-dependent postembryonic remodeling of differentiated tissues as a tadpole transforms into a frog. Rana [Lithobates] catesbeiana (American bullfrog) metamorphosis can be precociously induced in premetamorphic tadpoles by treatment with exogenous TH. However, metamorphosis is temperature-dependent and does not occur at 5°C, even with TH treatment. Remarkably, exposure to TH at 5°C establishes a molecular memory that primes the tadpole for an accelerated metamorphosis when shifted to a permissive temperature (24°C). Previous research suggests that histone post-translational modifications (PTMs) and the incorporation of histone variants are altered upon cold temperature exposure in the presence and absence of TH. Herein, we use mass spectrometry to analyze the histone composition of premetamorphic R. catesbeiana blood, liver, and tailfin following TH injection at a permissive temperature (24°C), a non-permissive temperature (5°C), and a shifted condition (5 to 24°C). We identify changes in histone and non-histone chromatin associated protein abundance and putative PTMs, suggesting that epigenetic variations may contribute to early TH signaling events and to molecular memory.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Lithobates Catesbeianus

TISSUE(S): Tail Fin, Liver, Blood

SUBMITTER: Haley Kuecks-Winger

LAB HEAD: Caren C.

PROVIDER: PXD062623 | Pride | 2025-04-25

REPOSITORIES: Pride

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			Action	DRS
	Blood-1.raw	Raw
	Blood-2.mzML	Mzml
	Blood-2.raw	Raw
	Bood-1.mzML	Mzml
	Liver-1.mzML	Mzml

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