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The source of dietary fat influences the immune response to syngeneic tumours in obesity – NK cell proteomic data

ABSTRACT: Obesity is linked to an increased risk of many cancers and can impair the anti-tumour immune response. This project examined the effect of different sources of dietary fats on tumour immunity using a syngeneic model of melanoma in mice fed high fat diets (HFD) derived from different sources. HFD based on beef tallow, lard and butter increased tumour growth in this model, while HFD derived from coconut oil, palm oil or olive oil did not. Further experiments focusing on butter and palm oil based HFD found differential regulation of natural killer (NK) cells and CD8 T-cell infiltration and function in the tumour microenvironment, and identified enrichment in immunosuppressive long chain acylcarnitine species in mice fed a HFD based on butter compared to palm oil. This dataset contains proteomic data from NK cells from mice fed a standard fat diet (SFD), NK cells from mice fed a HFD based on butter and from mice fed a HFD based on palm oil.

INSTRUMENT(S): Exactive Plus

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, Leukocyte

SUBMITTER: Christina Rollings

LAB HEAD: Lydia Lynch

PROVIDER: PXD062745 | Pride | 2025-06-03

REPOSITORIES: Pride

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Project description:An 8-week high fat palm oil diet causes obesity and insulin resistance in C57BL/6J mice, but induces only small changes in the muscle transcriptome. Introduction: The metabolic syndrome (MS) is a cluster of metabolic abnormalities linked to an increased risk of type 2 diabetes and cardiovascular diseases. Two major characteristics of the MS are obesity and insulin resistance. In the present study we investigated the effect of obesity and insulin resistance on the mouse muscle transcriptome. Methods: C57BL/6J mice were fed a palm oil-based low fat diet (LFD) or high fat diet (HFD) for eight weeks. Microarray analysis was performed by using two complementary strategies: (1) 8-week HFD transcriptome versus 8-week LFD transcriptome and (2) transcriptome of mice sacrificed at the start of the intervention versus 8-week LFD transcriptome and 8-week HFD transcriptome, respectively. Results: HFD mice develop obesity and whole-body insulin resistance. Despite these metabolic disturbances we found that HFD induces relatively small changes in gene expression (< 1.3 fold). Only the up-regulation of FA oxidation and the down-regulation of the MAPK cascade were specific for the HFD intervention. Eight-weeks of aging induced more changed gene expression levels than the HFD, including genes involved in cell-cell interaction and development. Conclusion: Eight weeks of aging induce more pronounced changes in the muscle transcriptome than an HFD. Since only one strategy revealed the transcriptional down-regulation of the MAPK cascade, whereas both strategies showed the up-regulation of FA oxidation we suggest the use of complementary analysis strategies by the genome-wide search of gene expression changes induced by mild interventions, such as an HFD. Keywords: diet intervention and time course In this study, C57BL/6J mice were fed a high fat (HF) diet for 8 weeks to induce obesity and insulin resistance. Plasma levels of the adipokines leptin and adiponectin were measured. To investigate how these metabolic disturbances will influence the skeletal muscle transcriptome we performed whole-genome microarray analysis. Functional implications were assessed by analyses of predefined gene sets based on Gene Ontology, biochemical, metabolic and signaling pathways.

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The source of dietary fat influences the immune response to syngeneic tumours in obesity – NK cell proteomic data

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