Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Phosphoproteomic Profiling of Acute Myocardial Infarction Serum Samples

ABSTRACT: Acute myocardial infarction (AMI) involves complex molecular events, including alterations in protein phosphorylation. In this study, we performed a comparative phosphoproteomic analysis of serum samples from AMI patients and healthy controls to identify AMI-associated phosphorylation signatures. Serum samples from eight AMI patients and two pooled controls underwent high-abundance protein depletion, enzymatic digestion with Trypsin/Lys-C, and phosphopeptide enrichment using Fe-NTA spin columns. Enriched phosphopeptides were analyzed by LC-MS/MS using a Q Exactive™ HF-X Orbitrap system.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Merve Oztug

LAB HEAD: Merve Oztug

PROVIDER: PXD062749 | Pride | 2026-05-25

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	2024_03_15_MI_Serum_Phosphoproteomics.mzTab	Mztab
	C1-1.raw	Raw
	C1-2.raw	Raw
	C2-1.raw	Raw
	C2-2.raw	Raw

Items per page:

1 - 5 of 22

Publications

Serum phosphoproteome alterations associated with cardiac troponin I levels in acute myocardial infarction.

Öztuğ Merve M Aşicioğlu Meltem M Altinkaynak Konca K Kilinç Evren E

Turkish journal of medical sciences 20260113 2

<h4>Background/aim</h4>Acute myocardial infarction (AMI) triggers systemic biochemical responses, including dynamic changes in the phosphorylation status of circulating proteins. However, the phosphoproteomic profile of serum in the context of AMI remains insufficiently characterized. This study aimed to investigate serum phosphoproteomic alterations associated with AMI and to explore potential correlations with markers of cardiac injury.<h4>Materials and methods</h4>A comparative phosphoproteom ...[more]

PMID: 42058992

Similar Datasets

Transciptome analysis of acute myocardial infarction

Project description:Analysis of peripheral blood specimens from patients with acute myocardial infarction (AMI). Results provide insight into molecular mechanisms associated with AMI.

2018-12-08 | GSE123487 | GEO

Blood milieu in acute myocardial infarction reprograms human macrophages for trauma repair

Project description:Acute myocardial infarction (AMI) is accompanied by a systemic trauma response that impacts on the whole body, including blood. In this study, we addressed whether macrophages, key players in trauma repair, sense and respond to these changes. For this, healthy human monocyte-derived macrophages were exposed to 20% human AMI (n=50) or control (n=20) serum and analyzed by transcriptional and multiparameter functional screening followed by network-guided data interpretation and drug repurposing. Results were validated in an independent cohort at functional level (n=47 AMI, n=25 control) and in a public dataset. AMI serum exposure resulted in an overt AMI signature, enriched in debris cleaning, mitosis, and immune pathways. Moreover, we identified gene networks associated with AMI and with poor clinical prognosis in AMI. Network-guided drug screening on the latter unveiled Prostaglandin E2 (PGE2) signaling as target for clinical intervention in detrimental macrophage imprinting during AMI trauma healing. Our results demonstrate pronounced context-induced macrophage reprogramming by the AMI systemic environment, to a degree decisive for patient prognosis. This offers new opportunities for targeted intervention and optimized cardiovascular disease risk management.

2022-12-18 | GSE215801 | GEO

Expression data from plasma of early-onset acute myocardial infarction patients and health control

Project description:CircRNAs are a new class of noncoding RNA and play regulatory roles in a variety of human diseases. However, the clinical significance of circRNAs in early-onset acute myocardial infarction (AMI) remains unknown. Therefore, we sought to explore the expression profile of circRNAs in early-onset AMI. Microarray was used to screen differentially expressed circRNAs between early-onset AMI patients and matched healthy controls.

2025-04-10 | GSE200572 | GEO

Reward System Activation Improves Recovery from Acute Myocardial Infarction".

Project description:The recovery from acute myocardial infarction (AMI) is influenced by physiological and psychological factors. While the physiological effects have been extensively studied, the contribution of psychological factors and whether they can be modulated is not completely clear. Here we show that the activity of the reward system, a brain network involved in motivation and positive expectation, affects outcomes in mice following AMI. We found that chemogenetic activation of dopaminergic neurons in the ventral tegmental area (VTA) significantly attenuated the remodeling process following AMI, resulting in improved left-ventricular performance compared to controls. VTA activation was associated with modulation of the immune response following AMI and increased blood vessel formation in the damaged area, mediated, at least in part, by changes in liver-secreted proteins. Together, our findings reveal a potential mechanism by which specific brain activity can affect the multifactorial systemic and local tissue response to AMI, introducing a novel target for therapeutic interventions

2024-05-03 | PXD043448 | Pride

Systemic environment in acute myocardial infarction programs human macrophages for trauma repair

Project description:In this study human primary monocyte-derived macrophages from healthy volunteers (pool of 6-8 donors) were exposed for 24 h to a 1:5 dilution of human patient serum. Patient sera were obtained either from control patients (n=20) or patients suffering an acute myocardial infarction (AMI, n=47, selected from POSTEMI trial, S. Limalanathan et al. J Am Heart Assoc 2014). All exposed macrophages were analyzed by RNA sequencing. The study revealed that macrophages significantly alter their gene expression profile in response the AMI-serum exposure, and adapt phagocytic, metabolic and cell cycling programs.

2022-12-18 | GSE172270 | GEO

Research on the circular RNA bioinformatics in patients with acute myocardial infarction

Project description:To further development of our gene expression approach to biodosimetry, We through the detection of circular RNA (circRNA) using expression profiling chips, we searched for circRNAs related to acute myocardial infarction (AMI) and explored their relationship and possible mechanisms with AMI. The study subjects included 3 AMI patients and 3 controls, and circRNA expression profiling analysis was performed using a microarray gene chip to identify circRNAs with large differences in expression between groups and to construct a circRNA-microRNA (circRNA-miRNA) network.

2022-04-22 | GSE149051 | GEO

Altered expression of microRNAs in the myocardium of rats with acute myocardial infarction

Project description:MicroRNAs are important cellular components and their dysfunctions are associated with various disease. Acute myocardial infarction (AMI) is one of the most serious cardiovascular diseases. Although several miRNAs have been reported to be associated with AMI, more novel miRNAs are needed to be investigated to ascertain if they are associated with AMI. SD rats (180-200g) was divided into sham-control group and two days group after AMI, seven days group after AMI, fourteen days group after AMI, each group has six individual animals total RNA was taken from the border-zone myocardium , low molecular weight RNA was seperate and labeled , and then hybridized to capitalbio V2 biochip representing about 924 microRNA . three chip were test in each group, and the procedure was repeated twice.

2010-04-22 | E-GEOD-19695 | biostudies-arrayexpress

Platelet gene expression profiling of acute myocardial infarction

Project description:Acute myocardial infarction (AMI) is primarily due to coronary atherosclerotic plaque rupture and subsequent thrombus formation. Platelets play a key role in the genesis and progression of both atherosclerosis and thrombosis. Since platelets are anuclear cells that inherit their mRNA from megakaryocyte precursors and maintain it unchanged during their life span, gene expression (GE) profiling at the time of an AMI provides information concerning the platelet GE preceding the coronary event. In ST-segment elevation myocardial infarction (STEMI), a gene-by-gene analysis of the platelet GE identified five differentially expressed genes (DEGs): FKBP5, S100P, SAMSN1, CLEC4E and S100A12. The logistic regression model used to combine the GE in a STEMI vs healthy donors score showed an AUC of 0.95. The same five DEGs were externally validated using platelet GE data from patients with coronary atherosclerosis but without thrombosis. Early signals of an imminent AMI are likely to be found by platelet GE profiling before the infarction occurs.

2019-12-31 | GSE109048 | GEO

Altered expression of microRNAs in the myocardium of rats with acute myocardial infarction

2010-01-05 | GSE19695 | GEO

Proteomic Comparison of Acute Myocardial Infarction and Stress Cardiomyopathy in Women

Project description:Background: Stress cardiomyopathy (SCM) is a unique form of LV dysfunction that more often occurs in women. Patients with SCM have a higher Troponin I/B-type natriuretic peptide ratio than AMI, but little is known about other circulating proteins. The goals of this study were to compare plasma proteins in SCM and AMI to learn about the pathophysiology of SCM and also to identify putative biomarkers of SCM. Methods: Blood was drawn in normal controls (n=6), women with AMI (n=12) or women with acute SCM (n=15). Two-week follow up samples were available in AMI (n=4) and SCM patients (n=11). Relative concentrations of 1310 serum proteins were measured in each of the 48 samples using the SOMAscan aptamer based assay. Women with AMI tended to be younger (57.87 ± 16.0 vs. 65.08 ± 9.11 years, p=0.12) and had a higher peak troponin I (AMI 32.03 ± 29.46 vs. SCM 2.68 ± 2.6 ng/mL, p=0.02). No differentially expressed proteins were detected (absolute log2 fold change>1; q<0.05) between AMI and SCM in the acute or recovery phase. In the normal vs. AMI comparison there was differential expression of 35 proteins. In the normal vs. SCM comparison there were 45 proteins with differential expression. Pathway analysis demonstrated activation of complement, coagulation, and inflammation in both AMI and SCM. Conclusions: The acute phase of SCM is characterized by a severe inflammatory response similar to AMI. Despite recovery of LV function in SCM at two weeks, differences in circulating proteins remain in comparison to normal controls.

2017-08-03 | GSE95368 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data