Characterization of Transcriptionally Active Clock Complexes in Time and Space Using Quantitative Proteomics
Ontology highlight
ABSTRACT: Circadian gene expression in mammals relies on the oscillatory activity of the BMAL1/CLOCK transcription factor complex, rhythmically binds to DNA. Despite the conserved presence of BMAL1/CLOCK in all tissues, circadian transcriptional outputs minimally overlap between tissues. We therefore hypothesize that BMAL1/CLOCK interact with tissue and time-dependent regulators at the chromatin generating an environment that determines tissue-specific gene expression. To investigate this, we combined chromatin immunoprecipitation with mass spectrometry-based quantitative proteomics (ChIP-MS) to characterize chromatin-bound BMAL1/CLOCK protein complexes at the transcriptional active phase (ZT4, ZT7, ZT10), in mouse tissues (Liver, Kidney, Lung). Together we produced and analyzed 108 pulldowns using BMAL1 and CLOCK specific antibodies. This large scale study yield a two dimensional (time & space) interaction map identifying known and unknown BMAL1/CLOCK interacting core proteins consistently present and time & tissue-specific complex constituents.
INSTRUMENT(S):
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Lung, Liver, Kidney
SUBMITTER:
Fatih Aygenli
LAB HEAD: Maria Robles
PROVIDER: PXD062751 | Pride | 2026-06-24
REPOSITORIES: Pride
ACCESS DATA