Proteomics

Dataset Information

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Deep analysis of FCS used in cell culture experiment


ABSTRACT: roteome analysis of commercially available foetal calf serum (FCS) for background correction. Commercially available FCS was depleted of high abundant and enriched for low abundant proteins with commercially available equalizer beads (BioRad) to create a background list of potential contaminates.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Klaus Kratochwill  

LAB HEAD: Dr Klaus Kratochwill

PROVIDER: PXD062778 | Pride | 2025-11-17

REPOSITORIES: Pride

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Publications

AQP7 deficiency drives adipose tissue remodeling and disrupts homeostasis.

Costa Ines Pd IP   Schiano Guglielmo G   Sacnun Juan Manuel JM   Herzog Rebecca R   Kerr Alastair A   Dahlman Ingrid I   Delporte Christine C   Kratochwill Klaus K   Devuyst Olivier O  

npj metabolic health and disease 20251107 1


The aquaporin-7 (AQP7) channel mediates glycerol release from adipocytes. Genetic variants decreasing AQP7 expression are associated with adiposity and metabolic complications in humans. Using human data, mouse models, and cellular systems, we investigated how AQP7 influences adipose tissue maturation and homeostasis. Negative correlations between methylation on the AQP7 locus, expression of AQP7 in the adipose tissue and BMI were observed in humans. Mice lacking Aqp7 had increased body weight a  ...[more]

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