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LC-MS by circHIPK3 probe method in human oral submucosal fibroblasts

ABSTRACT: To identify proteins to which circHIPK3 may bind and participate in the regulation of PDGF-BB-induced biological behavior in oral mucosal FBs, the circHIPK3 probe was designed for RNA pull-down experiment, and the proteins obtained from RNA pull-down were identified by silver staining, mass spectrometry and bioinformatics (COG, KEGG, PPI) analysis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Jie Wang

LAB HEAD: Jie Wang

PROVIDER: PXD062842 | Pride | 2025-05-13

REPOSITORIES: Pride

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Publications

PDGF-BB promotes oral submucosal fibrosis by driving phenotypic transformation and autophagy in oral mucosal fibroblasts through downregulation of circHIPK3.

Zhang Huamin H Zhou Yutong Y Jian Ni N Jiang Canhua C Wang Qi Q Wang Jie J

Scientific reports 20250502 1

Circular RNA HIPK3 (circHIPK3), known to regulate cell proliferation, migration, transformation, and autophagy in various fibrotic conditions. However, its role has not been studied in oral submucous fibrosis (OSF). Therefore, we conducted this study to explore whether platelet-derived growth factor-BB (PDGF-BB) induces human oral submucous fibroblasts (hOMF) proliferation, migration, transformation, and autophagy through circHIPK3 regulation. Treatment of hOMFs with PDGF-BB significantly increa ...[more]

PMID: 40316684

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