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SW480 cells were analyzed with RDA23B IP by mass spectrometry

ABSTRACT: SW480 cells were coprecipitated with RDA23B antibody and analyzed by mass spectrometry

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: jun li

LAB HEAD: Li Jun

PROVIDER: PXD062847 | Pride | 2025-11-10

REPOSITORIES: Pride

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			Action	DRS
	HD113GI.zip	Other
	HD113GI_IgG.raw	Raw
	HD113GI_RAD23BIP.raw	Raw
	checksum.txt	Txt

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Publications

RAD23B Promotes Colorectal Cancer Metastasis via the Talin1/Integrin/PI3K/AKT/MMP9 Axis.

Li Jun J Chen Yang Y Hao Zhijiao Z Zhang Zhiyong Z Fan Jingyi J Liu Xiao X Zhao Xueli X Zhang Hongyan H Wu Chenpeng C

Oncology research 20251022 11

<h4>Background</h4>Radiation sensitive 23 homolog B (RAD23B), a DNA repair-related protein, plays a contributory role in the development of multiple malignancies. This study aimed to explore the role of RAD23B in promoting colorectal cancer (CRC) metastasis and to elucidate the underlying molecular mechanisms.<h4>Methods</h4>RAD23B was overexpressed in CRC cell lines SW480 and HCT-8, with empty vectors serving as controls. Invasion, cell proliferation, and migration were assessed using CCK-8 and ...[more]

PMID: 41179295

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Project description:To validate the suitability of two commonly used colorectal cancer cell lines, DLD1 and SW480, as model systems to study colorectal carcinogenesis, we treated these cell lines with beta-catenin siRNA and identified beta-catenin target genes using DNA microarrays. The list of identified target genes was compared to previously published beta-catenin target genes found in the PubMed and the GEO databases. Based on the large number of beta-catenin target genes found to be similarly regulated in DLD1, SW480 and LS174T as well as the large overlap with confirmed β-catenin target genes, we conclude that DLD1 and SW480 colon carcinoma cell lines are suitable model systems to study beta-catenin regulated genes and signaling pathways 12 arrays (2 cell lines, 2 treatments, 3 biological replicates)

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Expression analysis of colorectal cancer cell line SW480 expressing FOXC2

Project description:Investigation of whole genome gene expression level changes in a colorectal cancer cell line SW480 expressing FOXC2, compared to the pBabe control cells. Genes associated with metastasis regulated by FOXC2 in colorectal cancer were analysed. The role of FOXC2 in breast cancer metastasis are further described in Mani SA, Yang J et al. Mesenchyme Forkhead 1 (FOXC2) plays a key role in metastasis and is associated with aggressive basal-like breast cancers. PNAS 2007; 104: 10069-10074 . A six chip study using total RNA recovered from three separate cultures of SW480/pBabe and three separate cultures of SW480/FOXC2. Each chip measures the expression level of 45033 genes from SW480/pBabe or SW480/FOXC2.

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MicroRNA expression profile between human colorectal cancer SW480 and SW620 cell line

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Expression data from SW480 cells with Gankyrin knockdown

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Human SW480 cells: Untreated vs H2O2 Treated

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SW480 Signaling Hub Inhibition

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