Dataset Information

Proteomic Characterization of the Rhesus Macaque Lens Nucleus: Similarity to Human Lens, Age Effects on Protein Solubility, and Trends in Posttranslational Modification

ABSTRACT: Proteomes of lens nuclei from young (4 years old) and old (15-16 years old) rhesus macaques (Macaca mulatta) were analyzed to determine similarity of the proteomic profile to that of human lenses, age-related differences in protein solubility, and association of various posttranslational modifications with age and protein solubility. Lens core proteins were separated into water-soluble and water-insoluble fractions using aqueous buffer and centrifugation. The water-insoluble fraction was solubilized using SDS. Proteins were processed using S-trap columns and peptide digests were analyzed using high-resolution, label-free DDA proteomics. Open modification searches were performed using MSFragger to identify possible PTMs. The number of modified peptide tandem mass spectra confidently assigned to samples by age or solubility were compared to find PTMs with statistically significant count differences. The overall proteomic profile of rhesus macaque lenses was very similar to human lenses, consisting of 80.2% crystallins, 1.1% beaded filament proteins, and 18.7% other proteins. The crystallin fraction consisted of 27% alpha crystallins, 67.6% beta/gamma crystallins, and 5.4% taxon-specific psi crystallin. Glycolytic enzymes, beta/gamma crystallins, and a few glutathione-related enzymes were found to have age-related shifts to the water-insoluble fraction. There were significant differences in deamidation, dioxidation, carbamylation, carboxymethylation, and trioxidation based on age and/or solubility of proteins.

INSTRUMENT(S):

ORGANISM(S): Macaca Mulatta (rhesus Macaque)

TISSUE(S): Lens Nucleus

SUBMITTER: Phillip Wilmarth

LAB HEAD: Jeremy Whitson, Ph.D.

PROVIDER: PXD062952 | Pride | 2025-09-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Submission_20250319.docx	Other
	UP000006718_9544_Rhesus_macaque_canonical_both.fasta	Fasta
	WHIT-1244_MSFragger-combined-protein.xlsx	Xlsx
	WHIT-1244_RhesusLens_14WIS_1ug_EasySpraySource_PSRThermotrapandcolumn_25cm_90min_20231122_QE.mgf	Mgf
	WHIT-1244_RhesusLens_14WIS_1ug_EasySpraySource_PSRThermotrapandcolumn_25cm_90min_20231122_QE.pepXML	Pepxml

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Proteomic Characterization of the Rhesus Macaque Lens Nucleus: Similarity to Human Lens, Age Effects on Protein Solubility, and Trends in Post-Translational Modifications.

Hayden Billy L BL Kelley Owen O Zientek Keith K Reddy Ashok P AP Wilmarth Phillip A PA Munds Rachel R Montague Michael J MJ Martinez Melween I MI Wollstein Gadi G Higham James P JP Barron Arrambide Arturo O AO Danias John J Melin Amanda D AD David Larry L LL Whitson Jeremy A JA

Investigative ophthalmology & visual science 20250901 12

<h4>Purpose</h4>Proteomes of lens nuclei from young (4 years old) and old (15-16 years old) rhesus macaques (Macaca mulatta) were analyzed to determine similarity of the proteomic profile to that of human lenses, age-related differences in protein solubility, and association of various post-translational modifications with age and protein solubility.<h4>Methods</h4>Lens core proteins were separated into water-soluble and water-insoluble fractions using aqueous buffer and centrifugation. The wate ...[more]

PMID: 40938073

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