Dataset Information

Heme acts as a molecular degrader linking CRL2-FEM1B to substrate proteolysis and ferroptosis regulation in lung cancer

ABSTRACT: In this project, we sought to identify the proteins interacting with the Elongin-C/Elongin-B complex. To this end, we performed affinity purification of Elongin-C and Elongin-B tagged with FLAG-STREP and MYC, respectively. A cell line with FLAG-STREP empty vector was used as negative control. 3 biological replicates were used for each sample group.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney Epithelial Cell

DISEASE(S): Lung Cancer

SUBMITTER: AUDEBERT Stephane

LAB HEAD: Luca Lignitto, PhD

PROVIDER: PXD062976 | Pride | 2026-06-11

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	ElonC_HeCe_Rep1.raw	Raw
	ElonC_HeCe_Rep1_20240322170829.raw	Raw
	ElonC_HeCe_Rep2.raw	Raw
	ElonC_HeCe_Rep2_20240322215621.raw	Raw
	ElonC_HeCe_Rep3.raw	Raw

Items per page:

1 - 5 of 14

Publications

CRL2FEM1B uses heme to recruit BACH1 for degradation and regulate ferroptosis in lung cancer.

Ahmed Bashir B Salaun Daniele D Jordan Jack B JB Daulat Avais A Pastorello Chiara C Brito Tarcísio T Yang Yuying Y Josselin Emmanuelle E Byrne Deborah D Betzi Stephane S Pelletier Amandine A Vernerey Julien J Ferreira Lorene L Castellano Remy R Audebert Stephane S Camoin Luc L Pane Attilio A Bechara Cherine C Borg Jean-Paul JP Modesti Mauro M Lignitto Luca L

Molecular cell 20260504 10

Lung cancers frequently increase iron demand to sustain growth, which makes them vulnerable to ferroptosis. While Cullin 2-RING ubiquitin ligases (CRL2s) are critical regulators of stress responses and redox balance, their role in ferroptosis mechanisms remains largely unknown. Here, we identify the E3 ligase CRL2FEM1B as a key regulator of the ferroptotic response. CRL2FEM1B recruits BTB and CNC homolog 1 (BACH1), a transcriptional regulator of ferroptosis, for degradation ...[more]

PMID: 42086045

Similar Datasets

Project description:The experiment was carried out during two vegetation seasons from 25th of April to 10th of September. Two Polish potato cultivars, the drought-tolerant Gwiazda and drought-sensitive Oberon were used. Selected tubers with transverse diameters of 3 - 4 cm were pre-sprouted for 2 weeks before planting. Plants were grown in a vegetation hall in pots filled with a thin layer of gravel on the bottom and the universal vegetable soil substrate ‘Hollas’ (Agaris Polska Ltd., Poland) produced from peat with the addition of chalk at a pH range of 5.5-6.5. Additionally, in phase 20 of the BBCH-scale of plant development, MIS-3 (Intermag) fertilizer was applied. Water content (WC) in volumetric basis in soil pots was measured according Black (1965). Weather conditions during the years of study were monitored using a Weather Campbell Station (Campbell Scientific Inc.) located in close proximity, and a thermohygrograph placed between pots. Meteorological data of air temperature, the photosynthetically active radiation, and humidity were comparable in the years of study and favorable for potato development. Three weeks after the initiation of the tuberisation phase (56 DAP), plants were divided into 3 groups, each consisting of 6 plants. The first group of plants was subjected to soil drought (remained without irrigation, day/night temperature 22°C/18°C), the second one to high temperature (day/night temperature 38°C/25°C) and the third one was watered according to needs and at optimal temperature (control plants, day/night temperature 22°C/18°C). Stress application lasted 14 days and finished at 70 DAP. During this period, plants were placed in phytotron equipped with six Hortilux Schreder Lamps with Philips light bulbs of 1600 W each. Air humidity was in the range 65-70%. WC under 14 days of soil drought reached 30% (v/v), and remained 80% (v/v) in control and high temperature conditions. During the recovery period, after 14 days of stress treatment, WC reached control levels. Plant root material for proteomic research was collected on the 14th day of stress treatment (70 DAP).

Dataset Information

Heme acts as a molecular degrader linking CRL2-FEM1B to substrate proteolysis and ferroptosis regulation in lung cancer

Dataset's files

Publications

CRL2<sup>FEM1B</sup> uses heme to recruit BACH1 for degradation and regulate ferroptosis in lung cancer.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Heme acts as a molecular degrader linking CRL2-FEM1B to substrate proteolysis and ferroptosis regulation in lung cancer

Dataset's files

Publications

CRL2&lt;sup&gt;FEM1B&lt;/sup&gt; uses heme to recruit BACH1 for degradation and regulate ferroptosis in lung cancer.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

CRL2<sup>FEM1B</sup> uses heme to recruit BACH1 for degradation and regulate ferroptosis in lung cancer.