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Lipids accumulation in nitrogen and phosphorus-limited yeast is caused by less growth-related dilution

ABSTRACT: Oleaginous yeasts are used commercially to produce oleochemicals and hold potential also for biodiesel production. In response to nitrogen or phosphorous limitation, oleaginous yeast accumulate triacylglycerol. Previous work has investigated potential mechanisms by which nutrient limitation induces lipid biosynthesis without verifying whether lipid biosynthesis flux is actually enhanced. Here we show, using 13C-glucose tracing, that in nitrogen or phosphorous limitation, lipid accumulation occurs without consistent increases in biosynthetic flux. Instead, the main driver of increased lipid pools is decreased growth-related dilution. This conclusion holds across two divergent oleaginous yeasts: Rhodotorula toruloides and Yarrowia lipolytica. Quantitative proteomics shows a substantial proteome reallocation in response to nitrogen and phosphorous limitation, with ribosomal proteins strongly down regulated, while lipid biosynthetic enzymes are preserved but not consistently upregulated in absolute quantity. Thus, nutrient limitation, rather than triggering greatly enhanced lipid synthesis, results in roughly sustained lipid enzyme levels and biosynthetic flux. Due to slower lipid dilution by cell division, this suffices to drive marked lipid accumulation.

INSTRUMENT(S):

ORGANISM(S): Yarrowia Lipolytica (candida Lipolytica) Rhodotorula Toruloides

SUBMITTER: Felix Keber

LAB HEAD: Martin Helmut Wühr

PROVIDER: PXD063019 | Pride | 2025-08-27

REPOSITORIES: Pride

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			Action	DRS
	ORC03362.raw	Raw
	ORC033620750973749realtimesearch.tsv	Tabular
	ORC03364.raw	Raw
	ORC033640750973749realtimesearch.tsv	Tabular
	ORC_03362_XL01RhoToru_RhoToru_TMTpro_20ppm_1iT_C125.mzXML	Mzxml

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