Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LC-MS/MS analysis of anti-LC3 products in human ccRCC cells

ABSTRACT: To identify proteins (especially E3 ligases) potentially binding to human LC3 protein, Caki-1 cells were used to perform LC-MS/MS analysis based on anti-LC3 (Proteintech, 14600-1-AP, 1:50) IP products (gel bands cutted after SDS-PAGE and CBB staining).Anti-IgG IP products were used as a negative control. Three independent experiments were performed.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Kidney

DISEASE(S): Kidney Cancer

SUBMITTER: Jing Song

LAB HEAD: Jing Song

PROVIDER: PXD063023 | Pride | 2025-04-16

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Search.xlsx	Xlsx
	antiIgG.raw	Raw
	antiLC3_rep1.raw	Raw
	antiLC3_rep2.raw	Raw
	antiLC3_rep3.raw	Raw

Items per page:

1 - 5 of 5

Similar Datasets

Mouse adult testes: Total RNA vs. IP RNA

2013-04-01 | GSE27582 | GEO

Mouse adult testes: Total RNA vs. IP RNA

Project description:RNAs that are enriched in AGO2 Immunoprecipitated (IP) products or PIWIL1 IP products were identified from mouse(BALB/C) adult testes by examine the ratio of total RNA signal intensity to AGO2 IP RNA or PIWIL1 IP RNA signal intensity. Two-condition experiment,Total RNA extracted from mouse adult testes vs. AGO2 IP RNA extracted from mouse adult testes and total RNA extracted from mouse adult testes vs. PIWIL1 IP RNA extracted from mouse adult testes.

2013-04-01 | E-GEOD-27582 | biostudies-arrayexpress

Project description:Taihu cbb functional gene

| PRJNA1103997 | ENA

LINC00955 Suppresses Colorectal Cancer Growth by Acting as a Molecular Scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated Methylation of the PHIP Promoter-nanoLCMS/MS analysis

Project description:To assess direct involvement of LINC00955 in regulation of gene expression, LINC00955 was used as an RNA probe in RNA pull-down assays and mass spectrometry analyses.

2023-10-24 | PXD041514 | Pride

Common bean resistance to Xanthomonas is associated with upregulation of the salicylic acid pathway and downregulation of photosynthesis.

Project description:Background: Common bacterial blight (CBB) caused by Xanthomonas phaseoli pv. phaseoli and Xanthomonas citri pv. fuscans is one of the major threats to common bean crops (Phaseolus vulgaris L.). Resistance to CBB is particularly complex as 26 quantitative resistance loci to CBB have been described so far. To date, transcriptomic studies after CBB infection have been very scarce and the molecular mechanisms underlying susceptibility or resistance are largely unknown. Results: We sequenced and annotated the genomes of two common bean genotypes being either resistant (BAT93) or susceptible (JaloEEP558) to CBB. Reciprocal BLASTp analysis led to a list of 20,787 homologs between these genotypes and the common bean reference genome (G19833), which provides a solid dataset for further comparative analyses. RNA-Seq after inoculation with X. phaseoli pv. phaseoli showed that the susceptible genotype initiated a more intense and diverse biological response than the resistant genotype. Resistance was linked to upregulation of the salicylic acid pathway and downregulation of photosynthesis and sugar metabolism, while susceptibility was linked to downregulation of resistance genes and upregulation of the ethylene pathway and of genes involved in cell wall modification. Conclusions: This study helps better understanding the mechanisms occurring during the early colonization phase of common bean by Xanthomonas and unveils new actors potentially important for resistance and susceptibility to CBB. We discuss the potential link between the pathways induced during bean colonization and genes induced by transcription activator-like effectors (TALEs), as illustrated in other Xanthomonas pathovars.

2020-07-27 | GSE155080 | GEO

MBOAT7 loss of function in clear cell Renal Carcinoma (ccRCC)

Project description:We demonstrated an increase in MBOAT7 and downstream phosphatidylinositol products in ccRCC. This RNAseq was of two loss of function clones and wild-type in the Caki-1 cell model (ccRCC cell line).

2020-02-26 | GSE131881 | GEO

Activation of autophagy by starvation and mTOR inhibition in three human cell lines

Project description:The aim of the project was to characterize changes in gene expression that are associated with induced autophagic flux. We engineered HeLa, HEK 293 and SH-SY5Y cell lines to express tandem-fluorecent LC3 (tf-LC3). The ratio of the red and green fluorophores indicated how much of the LC3 is in the acidic interior of lysosomes, which was a proxy measure for autophagic flux. RNA sequencing was performed for the cell lines at baseline and 1h, 15h and 30h after treatments. Additional untreated samples were also sequenced at some but not all time points. Autophagy was induced by amino acid starvation or mTOR inhibition (AZD8055).

2022-08-25 | E-MTAB-12020 | biostudies-arrayexpress

Histone exchange in wildtype, isw1, chd1, isw1 chd1 and ioc4 yeast strains

Project description:Histone exchange in wildtype, isw1, chd1, isw1 chd1 and ioc4 yeast strains. Two color experiment. Deletion mutant vs. WT cells. Biological replicates=3 per IP per cell type.

2012-07-24 | E-GEOD-32043 | biostudies-arrayexpress

Histone H3 K36 trimethylation in wild-type BY4741 yeast strains

Project description:ChIP-on chip assays to measure the change in histone H3 K36 trimethylation over the yeast genome in wild-type yeast strains. Two color experiment.WT cells. Biological replicates=3 per IP per cell type.

2012-07-24 | E-GEOD-37105 | biostudies-arrayexpress

Histone H3 K56 acetylation in wild-type histone shuffle strain (YBL574)

Project description:ChIP-on chip assays to measure the change in histone H3 K56 acetylation over the yeast genome in wild-type YBL574 yeast strains compared to H3K36A mutant strains. Two color experiment. Mutant vs WT cells. Biological replicates=3 per IP per cell type.

2012-07-24 | E-GEOD-37039 | biostudies-arrayexpress