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Design and Characterisation of Photoactivatable and Lysine Reactive Ortho Nitrobenzyl Alcohol-Based Crosslinkers

ABSTRACT: In this study, we performed a systematic analysis of ortho nitrobenzyl alcohol (oNBA) chemistry for integration into novel reagents for chemical crosslinking-mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine) Escherichia Coli

SUBMITTER: Antonio Calabrese

LAB HEAD: Antonio Calabrese

PROVIDER: PXD063033 | Pride | 2025-08-04

REPOSITORIES: Pride

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			Action	DRS
	24224_Ecl01_Anton_BS3_XL_TRAP_50cm_65min_BSA_hetero_1.raw	Raw
	24224_Ecl01_Anton_BS3_XL_TRAP_50cm_65min_BSA_hetero_1.zhrm	Other
	24224_Ecl01_Anton_BS3_XL_TRAP_50cm_65min_BSA_hetero_2.raw	Raw
	24224_Ecl01_Anton_BS3_XL_TRAP_50cm_65min_BSA_hetero_2.zhrm	Other
	24224_Ecl_DDA_IT500_TRAP_50cm_100ng_65min_BSA_oNBA_Methoxy_2.raw	Raw

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Publications

Design and characterisation of photoactivatable and lysine reactive o-nitrobenzyl alcohol-based crosslinkers.

Cahill Adam A Walko Martin M Fenton Benjamin B Ganji Sri Ranjani SR Herbert Anne A Radford Sheena E SE Kapur Nikil N Livingstone Keith K Wright Megan H MH Calabrese Antonio N AN

Chemical science 20250716 33

Photoreactive groups are invaluable tools in structural proteomics, offering reagent-free activation and temporal control of protein labelling. However, traditional UV-activatable functional groups often produce unstable intermediates and diverse products, making these chemistries difficult to deploy at scale. In this study, we performed a systematic analysis of ortho-nitrobenzyl alcohol (oNBA) reactivity for integration into novel reagents for chemical crosslinking-mass spectromet ...[more]

PMID: 40735685

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