Proteomics

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Proteomic characterisation of Japanese quail’s unique seminal foam


ABSTRACT: Seminal fluid plays an important role in fertilisation success, yet the molecular components of seminal fluid and their function remain poorly understood in most species. This study provides the first comprehensive proteomic characterisation of a unique seminal foam produced by the Japanese quail (Coturnix japonica), revealing its potential mechanistic function in sperm regulation, sperm motility, immune protection, and fertilisation success. By identifying key proteins associated with energy production, membrane fluidity, and sperm maturation, our findings suggest the foam may influence post-ejaculatory sperm function. Additionally, the identification of immunity proteins indicates a role in sperm protection and modulation of female immune responses. This study advances our understanding of avian reproductive biology and opens new avenues for investigating the specific role of seminal foam proteins in fertilisation dynamics and post-copulatory sexual selection.

INSTRUMENT(S):

ORGANISM(S): Coturnix Japonica

TISSUE(S): Sperm Flagellum

SUBMITTER: Trong Khoa Pham  

LAB HEAD: Trong Khoa Pham

PROVIDER: PXD063034 | Pride | 2026-03-23

REPOSITORIES: Pride

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Publications

Experimental Evidence That Prenatal and Postnatal Developmental Stress Affects the Adult Seminal Fluid Proteome in a Precocial Bird.

Mason Chloe C   Garlovsky Martin M   Vedder Oscar O   Pham Trong Khoa TK   George Rachel R   Tschirren Barbara B   Hemmings Nicola N  

Molecular ecology 20260101 2


Seminal fluid proteins are important modulators of male fertility and reproductive success, yet little is known about how their abundance responds to early-life developmental stress. Japanese quail Coturnix japonica) males produce a unique seminal foam that enhances fertilisation success. We characterised the proteome of the seminal foam for the first time and assessed how its composition is influenced by prenatal and postnatal developmental stress. Proteomic identification using liquid chromato  ...[more]

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