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The carnivorous marsh pitcher plants (Heliamphora sp.) secrete endogenous enzymes for prey digestion

ABSTRACT: The marsh pitcher plant (Heliamphora sp.) is a genus of carnivorous plant in which the production of endogenous digestive enzymes is still dubious. In this study, we tried to elucidate these old doubts and we investigated the ability of prey digestion in H. parva, H. neblinae and hybrid H. heterodoxa x minor. The plants were fed on fruit flies and digestive fluids were analysed using LC/MS, Western blots and enzyme activity measurements for presence of endogenous digestive enzymes. All investigated species produced endogenous digestive enzymes similar to those found in other carnivorous plant genera and the sister genus Sarracenia. Among these are: aspartic protease, subtilisin-like protease, type III chitinase, beta-1,3-glucanase, glucosidase, GDSL lipase, thaumatin-like protein etc. Phosphatase and proteolytic activities increased over time irrespective of feeding status. Immunoblotting of aspartic protease showed that abundance of the enzyme in digestive fluid can accumulate over time without any prey stimuli. The species of the genus Heliamphora can be considered as truly carnivorous plants with ability to produce endogenous digestive enzymes, although these are probably strongly diluted by rain in natural habitat, questioning their ecophysiological significance.

INSTRUMENT(S):

ORGANISM(S): Unclassified Heliamphora

TISSUE(S): Plant Cell, Digestive Juice

SUBMITTER: Ivo Chamrad

LAB HEAD: prof. Ondrej Novak, Ph.D.

PROVIDER: PXD063061 | Pride | 2026-08-03

REPOSITORIES: Pride

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			Action	DRS
	2024-09-02-decoys-contam-NCBI_DB_ERICALES_download_20240830.fasta.fas	Fasta
	Search_results.zip	Other
	TIM00846_ICH_AndrejP_NC1_3ul_BA1_1_4333.d.zip	Other
	TIM00846_ICH_AndrejP_NC1_3ul_BA1_1_4333_uncalibrated.mgf	Mgf
	TIM00847_ICH_AndrejP_4NC1_3ul_BA2_1_4334.d.zip	Other

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Project description:Direct comparison of the transcriptional patterns between male and female in the digestive gland of a natural population of the marine mussel Mytilus galloprovincialis sampled in the Bizerta Lagoon, Tunisia, across November 2007 -February March 2008 (four stages, winter peak). Background: Seasonal environmental changes may affect the physiology of Mytilus galloprovincialis (Lam.), an intertidal filter-feeder bivalve occurring commonly in Mediterranean and Atlantic coastal areas. We investigated seasonal variations in relative transcript abundance of the digestive gland and the Digestive gland (gonads) of males and females. To identify gene expression trends, we used a medium-density cDNA microarray (1.7 K probes) in dual-color competitive hybridization analyses. Results: Hierarchical clustering of digestive gland microarray data showed two main branches, distinguishing profiles associated with the “hot” months (May–August) from the other months. Genes involved in chitin metabolism, associated with mussel nutrition and digestion, showed higher expression during summer. Moreover, we found different gene expression patterns in the digestive glands of males and females during the four stages of mussel gonadal development. Microarray data from gonadal transcripts also displayed clear patterns during the different developmental phases with peak relative mRNA abundance at the ripe phase (stage III) for both sexes. Conclusion: These data showed a clear temporal pattern in gene expression profiles of mussels sampled over an annual cycle. Physiological response to thermal variation, food availability, and reproductive status across months may contribute to variation in gene expression. Test/reference design (female/male). Direct comparison of RNA extracts obtained from the Digestive gland tissue of female and male animals. Two (male, female) x four conditions (gonad developmental stage 1, stage 2, stage 3, stage 4). Dual color competitive hybridizations with label swap. Single individuals. Four biological replicates. One replicate per array.

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The carnivorous marsh pitcher plants (Heliamphora sp.) secrete endogenous enzymes for prey digestion

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