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TMT-based phosphoproteomics and proteomics analysis of spermatids

ABSTRACT: We characterized the dynamic protein phosphorylation profiles of spermatids across four different developing steps.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: xiangzheng zhang

LAB HEAD: xuejiang guo

PROVIDER: PXD063123 | Pride | 2025-12-08

REPOSITORIES: Pride

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			Action	DRS
	TMT16_mmsynchr_sperma_HPRP_20220708.rar	Other
	TMT16_mmsynchr_sperma_HPRP_F10_20220710.raw	Raw
	TMT16_mmsynchr_sperma_HPRP_F11_20220713.raw	Raw
	TMT16_mmsynchr_sperma_HPRP_F12_20220713.raw	Raw
	TMT16_mmsynchr_sperma_HPRP_F13_20220714.raw	Raw

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Publications

Stage-Resolved Phosphoproteomic Landscape of Mouse Spermiogenesis Reveals Key Kinase Signaling in Sperm Morphogenesis.

Zhu Tianyu T Zhu Yan Y Jiang Xinyi X Zhang Xiangzheng X Wang Bing B Chen Yu Y Zhao Yichen Y Wang Yulian Y Zhou Qi Q Han Zhongyan Z Qi Yaling Y Luo Mengjiao M Tu Haixia H Hao Bingjie B Gao Mengmeng M Ren Jie J Zhou Xin X Zhang Xingyu X Chen Xu X Li Haojie H Huang Qian Q Situ Chenghao C Guo Yueshuai Y Zhu Hui H Li Yan Y Guo Xuejiang X

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20250903 44

Spermiogenesis is the committed step of sperm production, during which spermatid cells undergo dramatic morphological transformations and transcriptional silencing. Post-translational modifications (PTMs), including phosphorylation, provide a level of protein function flexibility and play important roles in spermiogenesis. Dynamic protein phosphorylation profiles of spermatids are characterized across four different developing steps, and identified phosphorylation regulation of key proteins in s ...[more]

PMID: 40903803

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