Nucleo-cytoplasmic fractionation MS for proteins mislocalized with cytoplasmic TDP-43 in human iPSC-derived neurons

ABSTRACT: We aimed to unbiasedly identify proteins mislocalizing with cytoplasmic TDP-43 from the nucleus to the cytoplasm. We therefore expressed TDP-43deltaNLS or nuclear export signal (NES) as cytoplasmic control in induced neurons and performed cellular fractionation, separating nuclear from cytoplasmic fractions, before doing MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Welmoed van Zuiden  

LAB HEAD: Eran Hornstein

PROVIDER: PXD063188 | Pride | 2026-10-15

REPOSITORIES: Pride