Dataset Information

Proximity labeling of dense core vesicle protein VMAT

ABSTRACT: Neuropeptide-containing dense core vesicles (DCVs) are generated in the neuronal cell body and circulate throughout the axonal arbor to supply distal release sites. This circulation depends on the anterograde kinesin-1 and kinesin-3 motors and the retrograde dynein-dynactin motor, however the adaptor proteins involved are poorly understood. In addition to their luminal neuropeptide cargo DCVs also contain specific transmembrane proteins including the vesicular monoamide transporter (VMAT). To identify potential components of DCV motor adaptor complexes in Drosophila neurons we performed proximity labeling based on a pan-neuronally expressed TurboID-VMAT chimera, as well as a free cytosolic TurboID used as a negative control. This approach may also shed light on the trafficking pathways involved in sorting VMAT and other membrane proteins to DCVs.

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Whole Body

SUBMITTER: Michael Wierer

LAB HEAD: Ole Kjaerulff

PROVIDER: PXD063200 | Pride | 2026-01-12

REPOSITORIES: Pride

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Publications

A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles.

Lund Viktor Karlovich VK Chirco Antony A Caliari Michela M Larsen Andreas Haahr AH Tang Kristoffer Tollestrup KT Gether Ulrik U Madsen Kenneth Lindegaard KL Wierer Michael M Kjaerulff Ole O

The Journal of cell biology 20260106 3

Neuropeptide-containing dense core vesicles (DCVs) generated in neuronal somata are circulated in axons to supply distal release sites, depending on kinesin-1, kinesin-3, and dynein, but how the motors are recruited remains unclear. Here we use proximity proteomics in the living Drosophila nervous system to identify the protein complex responsible for recruitment of kinesin-1 and dynein on DCVs. We find that the dynein and kinesin-1 adaptor Sunday driver (Syd/dJIP3/4) interact with the DCV-locat ...[more]

PMID: 41493273

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