Dataset Information

Spatial Proteomics Profiling of Vascular Smooth Muscle Cells and Atherosclerotic Plaques in a Diabetic Atherosclerosis Mouse Model

ABSTRACT: This project presents a spatial proteomic investigation of vascular smooth muscle cells (VSMCs) and atherosclerotic plaque regions in a diabetic mouse model of atherosclerosis (DMAS). Using ApoE-/- mice fed with a high-fat diet and treated with low-dose streptozotocin (STZ) to induce a diabetic phenotype, we performed laser capture microdissection (LCM) to separately isolate plaque regions and adjacent VSMC-rich areas of the aortic arch. Subsequently, spatially resolved proteomic profiling was carried out using LC-MS/MS, followed by quantitative analysis based on iBAQ values. Differentially expressed proteins between plaque and VSMC regions were identified and enriched pathway analysis revealed significant alterations in oxidative stress response, insulin signaling, mitochondrial function, RNA transport, and extracellular matrix remodeling under diabetic conditions. Of particular interest, the spatial proteomics data highlighted a marked downregulation of the nuclear pore complex protein Nup93 in VSMCs from diabetic mice, implicating impaired nuclear transport and dysregulation of RNA alternative splicing. This finding was supported by transcriptomic integration and functional analyses that revealed aberrant splicing of SerpinE2, a gene associated with cell proliferation and ECM accumulation. This dataset provides region-specific, high-resolution proteomic profiles of vascular tissues in a diabetic atherosclerosis model. It serves as a valuable resource for investigating the spatial heterogeneity of vascular pathology and the molecular mechanisms underlying diabetes-accelerated atherosclerosis. The raw and processed data can be used to explore protein expression dynamics, identify spatiotemporal biomarkers, and validate candidate targets such as Nup93 and SerpinE2 involved in VSMC phenotypic switching.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Aortic Arch

SUBMITTER: Yuan Xiaojing

LAB HEAD: Xiaojing Yuan

PROVIDER: PXD063231 | Pride | 2025-09-29

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	RE730SD_AS_mc2_C3_1_20250.d.zip	Other
	RE730SD_AS_mc3_C4_1_20252.d.zip	Other
	RE730SD_AS_p1_B4_1_20236.d.zip	Other
	RE730SD_AS_p2_B5_1_20238.d.zip	Other
	RE730SD_DMAS_mc1_C5_1_20254.d.zip	Other

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Publications

Nup93-Mediated RNA Alternative Splicing Associated With Diabetic Atherosclerosis.

Yuan Xiaojing X Zhang Qilun Q Li Jie J Zhang Zichen Z Ye Shandong S Zhou Wan W

Molecular & cellular proteomics : MCP 20250707 9

Atherosclerosis, a life-threatening complication of diabetes mellitus (DM), significantly increases the mortality risk among diabetic patients. Vascular smooth muscle cells (VSMCs) not only constitute the core of atherosclerotic lesions but also serve as primary components of plaques. Although diabetes expedites this transformation process, the specific mechanism remains elusive. Traditional proteomic approaches that analyze average signals of all cells overlook the importance of spatial informa ...[more]

PMID: 40633766

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