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E3 activity-based proteomic profiling of HEK293 cells treated with ATPgammaS

ABSTRACT: RNF213 is novel transthiolating E3 ligase that is activated by ATP. To determine whether cellular RNF213 is activated by elevated ATP levels, we electroplated an E3 activity-based probe into HEK293 cells stably expressing RNF213 in a knockout background. DIA analysis was performed in cells that were coelectroporated with the poorly hydrolysable ATP analog, ATPgS, or buffer control.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Frederic Lamoliatte

LAB HEAD: Satpal Virdee

PROVIDER: PXD063291 | Pride | 2025-04-25

REPOSITORIES: Pride

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			Action	DRS
	20221028_FL_Lu_SV_Set12_A1.raw	Raw
	20221028_FL_Lu_SV_Set12_A2.raw	Raw
	20221028_FL_Lu_SV_Set12_A3.raw	Raw
	20221028_FL_Lu_SV_Set12_A4.raw	Raw
	20221028_FL_Lu_SV_Set12_A5.raw	Raw

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