Proteomics

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Quantitative proteomic analysis of the Toxoplasma gondii cytoskeleton and bioinformatic identification of highly antigenic proteins

ABSTRACT: The Toxoplasma gondii cytoskeleton is a highly organized structure essential for parasite motility, replication, and host cell invasion. To identify its components, a highly enriched fraction of tachyzoite cytoskeletons was obtained and quantitatively analyzed by mass spectrometry (LC/ESI/HDMSE). We identified 623 proteins classified into 18 functional groups, including 21 IMC proteins, 26 cytoskeleton proteins, and 42 uncharacterized proteins. A comprehensive bioinformatic analysis was conducted to assess protein abundance (fmol), antigenicity, accessibility, interactome, and homology, with the aim of identifying immunogenic targets. Among the top vaccine candidates were GRA12, IMC1, ROP8, and IMC4, with ROP8 emerging as the most promising based on epitope prediction.

INSTRUMENT(S):

ORGANISM(S): Eukaryota Toxoplasma Gondii Rh

DISEASE(S): Toxoplasmosis

SUBMITTER: Karina Araujo  

LAB HEAD: Ricardo Mondragón-Flores

PROVIDER: PXD063409 | Pride | 2025-08-04

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20181112_HDMSE_CK1.raw.rar Raw
20181112_HDMSE_CK1_IA_final_peptide.csv Csv
20181112_HDMSE_CK1_IA_final_peptide_Toxo_DB.csv Csv
20181112_HDMSE_CK1_IA_final_protein.csv Csv
20181112_HDMSE_CK1_IA_final_protein_ToxoDB.csv Csv
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Publications

The Toxoplasma gondii cytoskeleton is a highly organized structure essential for parasite motility, replication, and host cell invasion. To identify its components, a highly enriched fraction of tachyzoite cytoskeletons was obtained and quantitatively analyzed by mass spectrometry. We identified 623 proteins classified into 18 functional groups, including 30 IMC proteins, 34 cytoskeleton proteins, and 14 uncharacterized proteins. A comprehensive bioinformatic analysis was conducted to assess pro  ...[more]

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