Proteomics

Dataset Information

0

Phospho-Peptide Pull-Down Reveals Inhibitory Signaling Partners of CD200R1


ABSTRACT: Project Description (Revised): CD200R1 inhibits phagocytosis through its intracellular tyrosine residues Y286 and Y297. To investigate whether these residues recruit inhibitory molecules, we performed pull-down assays using synthetic peptides—either phosphorylated or unphosphorylated—corresponding to regions containing Y286 and Y297. These peptides were incubated with lysates from mouse bone marrow-derived macrophages (BMDMs), and interacting proteins were subsequently identified by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Jiaxin Li  

LAB HEAD: Jiaxin Li and Andre Veillette

PROVIDER: PXD063538 | Pride | 2025-09-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
OF_20220311_VEI_01.raw Raw
OF_20220311_VEI_01_0.raw Raw
OF_20220311_VEI_04.raw Raw
OF_20230823_VEI_01.raw Raw
OF_20230823_VEI_01_0.raw Raw
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