Quantification of protein level changes under elevated mitochondrial abasic site damage

ABSTRACT: The project aims to examine the protein level changes under induced mitochondrial abasic site lesions in HeLa cells using stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics. The data revealed significant changes in the expression level of several proteins. A decrease in mitochondrial ribosomal proteins (MRPS35, MRPL15, MRPL37, MRPL13, and MRPL4) and complex I subunits (NDUFA12 and NDUFA13) was observed, suggesting a suppression of mitochondrial protein synthesis and energy production under these conditions. In addition, the levels of ARF5 (ADP ribosylation factor 5), DNM1L (dynamin-1-like protein), and PRDX6 (peroxiredoxin 6) were upregulated. ARF5 is involved in protein trafficking, and DNM1L mediates mitochondrial and peroxisomal division.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Hela Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Yu Hsuan Chen  

LAB HEAD: Linlin Zhao

PROVIDER: PXD063576 | Pride | 2025-12-03

REPOSITORIES: Pride