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Proteomic characterization of brucellin: identifying key immunoreactive proteins for improved brucellosis diagnosis

ABSTRACT: This study used nLC-ESI-MS/MS analysis and bioinformatics tools to evaluate brucellin's protein composition and identify immunoreactive proteins.

INSTRUMENT(S):

ORGANISM(S): Brucella Melitensis B115

TISSUE(S): Cell Culture

SUBMITTER: Federica D'Onofrio

LAB HEAD: Mirella Luciani

PROVIDER: PXD063758 | Pride | 2026-02-09

REPOSITORIES: Pride

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			Action	DRS
	BRUCELLINA-ANSES-2_240503170112.mzML	Mzml
	BRUCELLINA-ANSES-3.mzML	Mzml
	BRUCELLINA-OCB-1_240426052750.mzML	Mzml
	BRUCELLINA-OCB-2_240426071236.mzML	Mzml
	BRUCELLINA-OCB-3_240426085721.mzML	Mzml

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Publications

Identifying immunoreactive proteins in brucellin for enhanced brucellosis diagnosis: a proteomic approach.

Krasteva Ivanka I Luciani Mirella M D'Onofrio Federica F Di Febo Tiziana T Di Pancrazio Chiara C Perletta Fabrizia F Maggetti Marta M Ulisse Simonetta S Sonsini Luigina L Orsini Gianluca G Caporale Marco M Ponsart Claire C Djokic Vitomir V Vicente Acacia Ferreira AF Freddi Luca L D'Alterio Nicola N Tittarelli Manuela M De Massis Fabrizio F Sacchini Flavio F

Frontiers in microbiology 20250730

The brucellin skin test (BST) detects brucellosis in animals through a cell-mediated immune response to a protein extract from <i>B. melitensis</i> strain 115, which is almost free of lipopolysaccharide. It is highly specific and used to confirm suspected false positive serology results in small ruminants and swine, but not recommended for screening due to low sensitivity. Despite its diagnostic significance, the protein composition of brucellin has not been fully characterized. This study used ...[more]

PMID: 40809042

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SLC19A1 transports immunoreactive cyclic dinucleotides.

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