BODY FLUID PROTEOMIC LANDSCAPE OF ACUTE EXERCISE

ABSTRACT: Physical activity improves health, yet the molecular mechanisms underlying its systemic benefits remain incompletely understood. Here, we present a high-resolution, time-resolved atlas of 10,127 proteins, identifying over 3,000 that changed across plasma, saliva, and urine following acute exercise in healthy adults. By integrating deep proteomic profiling with tissue enrichment, disease associations, and network analysis, we delineate fluid-specific, temporally distinct responses linked to various tissues and diseases. We elucidated coordinated remodeling in the extracellular matrix, protein homodimerization activity, and immune activation orchestrating exercise-induced networks. Most plasma responses were robust across age, sex and exercise modality, indicating a conserved systemic signature. Genetic colocalization analysis established exercise-regulated proteins as modulators of metabolic traits and identified over 200 proteins targeted by FDA-approved drugs, highlighting opportunities for therapeutic repurposing. This comprehensive molecular atlas yields mechanistic insights into exercise adaptation pathways and constitutes a valuable resource for advancing exerkine discovery, biomarker development, and pharmacological exercise-mimetic strategies.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine, Blood Plasma, Saliva

SUBMITTER: Nigel Kurgan  

LAB HEAD: Atul Shahaji Deshmukh

PROVIDER: PXD063954 | Pride | 2026-10-26

REPOSITORIES: Pride