Thermal Proteome Profiling (TPP) of l-Cysteine–Treated Arabidopsis Mitochondrial Proteins
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ABSTRACT: This project investigates the thermal stability of isolated mitochondrial proteins from Arabidopsis thaliana cell cultures following L-cysteine treatment, using Thermal Proteome Profiling (TPP). Shotgun proteomics was employed to identify and quantify proteins after thermal treatment. Quantitative data (iBAQ values) were subsequently analyzed using a custom R script (available on GitHub: “MeCuP”) to identify candidate proteins that exhibit changes in thermal stability due to interaction with L-cysteine.
INSTRUMENT(S): timsTOF HT
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)
TISSUE(S): Cell Culture
SUBMITTER:
Björn Heinemann
LAB HEAD: Prof. Dr. Tatjana Hildebrandt
PROVIDER: PXD064096 | Pride | 2025-05-29
REPOSITORIES: Pride
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