Proteomics

Dataset Information

0

Thermal Proteome Profiling (TPP) of l-Cysteine–Treated Arabidopsis Mitochondrial Proteins


ABSTRACT: This project investigates the thermal stability of isolated mitochondrial proteins from Arabidopsis thaliana cell cultures following L-cysteine treatment, using Thermal Proteome Profiling (TPP). Shotgun proteomics was employed to identify and quantify proteins after thermal treatment. Quantitative data (iBAQ values) were subsequently analyzed using a custom R script (available on GitHub: “MeCuP”) to identify candidate proteins that exhibit changes in thermal stability due to interaction with L-cysteine.

INSTRUMENT(S): timsTOF HT

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Cell Culture

SUBMITTER: Björn Heinemann  

LAB HEAD: Prof. Dr. Tatjana Hildebrandt

PROVIDER: PXD064096 | Pride | 2025-05-29

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MeCuP_DEMO__Mito_Cys3__treat__Filtered.zip Other
TPP_raw.files.zip Other
TPP_sample_list.xlsx Xlsx
combined_txt.zip Other
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