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Proteomic insights in Acrocomia aculeata: Optimizing sample preparation techniques for analysis of an oleaginous alternative crop

ABSTRACT: Proteomic analysis of non-model plants requires selecting appropriate sample preparation techniques to reliable and comprehensive results. Acrocomia aculeata, a high-yielding, adaptable palm native to Tropical America, poses proteomic analysis challenges due to its undomesticated status and genetic variability. To cope withthis issue, we applied different sample preparation protocols for the proteomics analysis of A. aculeata endosperm and mesocarp tissues.

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ORGANISM(S): Acrocomia Aculeata

TISSUE(S): Mesocarp, Endosperm

SUBMITTER: Proteomics Unit

LAB HEAD: Fábio César Sousa Nogueira

PROVIDER: PXD064115 | Pride | 2026-07-07

REPOSITORIES: Pride

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Project description:Here we exploit the essential process of X-chromosome dosage compensation to elucidate basic mechanisms that control the assembly, genome-wide binding, and function of gene regulatory complexes that act over large chromosomal territories. We demonstrate that a subunit of C. elegans MLL/COMPASS, a gene-activation complex, acts within the dosage compensation complex (DCC), a condensin complex, to target the DCC to both X chromosomes of hermaphrodites and thereby reduce chromosome-wide gene expression. The DCC binds to two categories of sites on X: rex sites that recruit the DCC in an autonomous, sequence- dependent manner, and dox sites that reside primarily in promoters of expressed genes and bind the DCC robustly only when attached to X. We find that DCC mutants that abolish rex-site binding do not eliminate dox-site binding, but instead reduce it to the level observed at autosomal binding sites in wild-type animals. Changes in DCC binding to these non-rex sites occur throughout development and correlate with transcriptional activity of adjacent genes. Moreover, autosomal DCC binding is enhanced by rex-site binding in cis in X-autosome fusion chromosomes. Thus, dox and autosomal sites exhibit similar binding properties. Our data support a model for DCC binding in which low-level DCC binding at dox and autosomal sites is dictated by intrinsic properties correlated with high transcriptional activity. Sex-specific DCC recruitment to rex sites then greatly elevates DCC binding to dox sites in cis, which lack intrinsically high DCC affinity on their own. We also show here that the C. elegans DCC achieves dosage compensation through its effects on transcription. ChIP-chip experiments using antibodies against DPY-27, SDC-3, DPY-30, DPY-26, MIX-1, SMC-4, ASH-2 in wild-type embryos. ChIP-chip experiments using antibodies against SDC-3, DPY-27, DPY-30, ASH-2, and IgG in different DCC mutants. ChIP-chip experiments using antibodies against RNA Pol II (hypophosphorylated and S2 and S5) in wild type and sdc-2 partial loss of function mutants.

			Action	DRS
	OE00508_EMGF_1_1.raw	Raw
	OE00509_EMGF_1_2.raw	Raw
	OE00510_EMGF_2_1.raw	Raw
	OE00511_EMGF_2_2.raw	Raw
	OE00512_EMGF_3_1.raw	Raw

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Proteomic insights in Acrocomia aculeata: Optimizing sample preparation techniques for analysis of an oleaginous alternative crop

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