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Proteomics analysis of SDE2UBL base activity based probe immunoprecipitation

ABSTRACT: SDE2, a highly conserved and essential gene required for genome maintenance, coordinates essential processes ranging from pre-mRNA splicing and ribosome biogenesis to DNA repair and replication stress response. It is expressed as a full-length precursor (SDE2FL,) bearing an N-terminal ULD, which is proteolytically cleaved to generate two protein fragments, SDE2UBL and the larger C-terminal domain (SDE2CT). Here we report the use of activity-based probe to aid the identification of the protease responsible for SDE2 processing, resolving a previously uncharacterized mechanism of post-translational regulation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Frederic Lamoliatte

LAB HEAD: Chiara Maniaci

PROVIDER: PXD064155 | Pride | 2026-06-24

REPOSITORIES: Pride

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			Action	DRS
	CManiaci_20240719_01.raw	Raw
	CManiaci_20240719_02.raw	Raw
	CManiaci_20240719_03.raw	Raw
	CManiaci_20240719_04.raw	Raw
	CManiaci_20240719_05.raw	Raw

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Project description:SDE2, a highly conserved and essential gene required for genome maintenance, coordinates essential processes ranging from pre-mRNA splicing and ribosome biogenesis to DNA repair and replication stress response. It is expressed as a full-length precursor (SDE2FL,) bearing an N-terminal ULD, which is proteolytically cleaved to generate two protein fragments, SDE2UBL and the larger C-terminal domain (SDE2CT). In this study we report the identification of the protease responsible for SDE2 processing, resolving a previously uncharacterized mechanism of post-translational regulation.

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