Dataset Information

IP-MS experiment for the ubiquitin-conjugating E2 enzyme SiUBC39

ABSTRACT: In Setaria italica (foxtail millet), SiUBC39 is implicated in regulating key agronomic traits, including plant height, flowering time, and stress tolerance. To elucidate the molecular mechanisms underlying SiUBC39’s roles in these traits, we affinity-purified SiUBC39-GFP and the corresponding GFP control protein via immunoprecipitation (IP). The immunoprecipitated complexes were then subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to identify proteins interacting with SiUBC39.

INSTRUMENT(S):

ORGANISM(S): Setaria Italica

TISSUE(S): Leaf

SUBMITTER: Tang Sha

LAB HEAD: Sha Tang

PROVIDER: PXD064229 | Pride | 2025-09-04

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 9

Similar Datasets

Project description:Background Extracellular vesicles (EVs) secreted by mesenchymal stromal cells (MSCs) provide significant protection against renal ischemia-reperfusion injury (IRI). Hypoxia is considered an important method for enhancing the tissue repair capabilities of MSCs. However, the specific effects of hypoxia on MSCs and MSC-EVs, as well as their therapeutic potential for renal IRI, remain unclear. In this study, we investigated the alterations in MSCs and the production of MSC-EVs following hypoxia pre-treatment, and further explored the key intrinsic mechanisms by which hypoxic MSC-EVs treat renal IRI. Methods Human umbilical cord MSCs were cultured under normoxic and hypoxic conditions. Proliferation and related pathways were measured, and RNA sequencing was used to detect changes in the transcription profile. MSC-EVs from both normoxic and hypoxic conditions were isolated and characterized. In vivo, the localization and therapeutic effects of MSC-EVs were assessed in a rat renal IRI model. Histological examinations were employed to assess the structure, proliferation, and apoptosis of IRI kidney tissue respectively. Renal function was measured by analyzing serum creatinine and blood urea nitrogen levels. In vitro, the therapeutic potential of MSC-EVs were measured in renal tubular epithelial cells injured by antimycin A. Protein sequencing analysis of hypoxic MSC-EVs was conducted, and the depletion of Glutathione S-Transferase Omega 1 (GSTO1) in hypoxic MSC-EVs was performed to verify its key role in alleviating renal injury. Results Hypoxia alters MSCs transcription, promotes their proliferation, and increases the production of EVs. Hypoxia-pretreated MSC-EVs exhibited a superior ability to mitigate renal IRI, enhancing proliferation and reducing apoptosis of renal tubular epithelial cells both in vivo and in vitro. Protein profiling of the EVs revealed an accumulation of numerous anti-oxidative stress proteins, with GSTO1 being particularly prominent. GSTO1 knock down was significantly reduced the antioxidant and therapeutic effects in renal IRI of hypoxic MSC-EVs. Conclusions Hypoxia significantly promotes MSC-EVs generation and enhances the therapeutic effect of EVs on renal IRI. The effect of antioxidant stress induced by GSTO1 is one of the most important underlying mechanisms. Our findings underscore that hypoxia-pretreated MSC-EVs represent a novel and promising therapeutic intervention for renal IRI.

			Action	DRS
	CK_Results.zip	Other
	E2_1_Results.zip	Other
	E2_2_Results.zip	Other
	E2_3_Results.zip	Other
	XC02631DI_CK.raw	Raw

Dataset Information

IP-MS experiment for the ubiquitin-conjugating E2 enzyme SiUBC39

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets