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Labelfree proteomics in Extracellular vesicles from human microglia cells

ABSTRACT: Label-free proteomics is used to study the role of the intracellular sorting receptor SORLA in the formation of functional exosomes by iPSC-derived human microglia.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Johan Palmfeldt

LAB HEAD: Johan Palmfeldt

PROVIDER: PXD064297 | Pride | 2025-09-11

REPOSITORIES: Pride

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			Action	DRS
	EV-Knockout-1.raw	Raw
	EV-Knockout-2.raw	Raw
	EV-Knockout-3.raw	Raw
	EV-Mutant-1.raw	Raw
	EV-Mutant-2.raw	Raw

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1 - 5 of 11

Publications

Familial Alzheimer's disease mutation identifies novel role of SORLA in release of neurotrophic exosomes.

Juul-Madsen Kristian K Rudolph Ina-Maria IM Gomes Jemila P JP Meyer Katrina K Ovesen Peter L PL Gorniak-Walas Malgorzata M Kokoli Marianna M Telugu Narasimha S NS von Tangen Sivertsen Malthe M Febbraro Fabia F Sutherland Duncan S DS Palmfeldt Johan J Diecke Sebastian S Andersen Olav M OM Selbach Matthias M Willnow Thomas E TE

Alzheimer's & dementia : the journal of the Alzheimer's Association 20250901 9

<h4>Introduction</h4>Mutations in SORL1, encoding the sorting receptor Sortilin-related receptor with A-type repeats (SORLA), are found in individuals with Alzheimer's disease (AD). We studied SORLA<sup>N1358S</sup>, carrying a mutation in its ligand binding domain, to learn more about receptor functions relevant for human brain health.<h4>Methods</h4>We investigated consequences of SORLA<sup>N1358S</sup> expression in induced pluripotent stem cell (iPSC)-derived human neurons and microglia, usi ...[more]

PMID: 40928027

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