Establishing a MS-based assay for in vivo peptide deformylase inhibition in Bacillus subtilis

ABSTRACT: The peptide deformylase inhibitor actinonin was used to treat Bacillus subtilis 168, and the proteome was analyzed using UPLC-MSE. The goal was to identify proteins that retain N-terminal formylation of the initial methionine after actinonin treatment that could be used to establish a microplate assay based on mass spectrometry for in vivo peptide deformylase inhibition.

INSTRUMENT(S):

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Sina Schäkermann  

LAB HEAD: Julia E. Bandow

PROVIDER: PXD064376 | Pride | 2026-02-11

REPOSITORIES: Pride