Establishing a MS-based assay for in vivo peptide deformylase inhibition in Bacillus subtilis
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ABSTRACT: The peptide deformylase inhibitor actinonin was used to treat Bacillus subtilis 168, and the proteome was analyzed using UPLC-MSE. The goal was to identify proteins that retain N-terminal formylation of the initial methionine after actinonin treatment that could be used to establish a microplate assay based on mass spectrometry for in vivo peptide deformylase inhibition.
INSTRUMENT(S):
ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168
SUBMITTER:
Sina Schäkermann
LAB HEAD: Julia E. Bandow
PROVIDER: PXD064376 | Pride | 2026-02-11
REPOSITORIES: Pride
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