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Identification of binding partners of the purified TOM complex from HEK293T cells

ABSTRACT: To investigate the interactome of the human translocase of the outer mitochondrial membrane (TOM) complex, we performed immunoaffinity purification of TOMM22-FLAG from HEK293T cells followed by LC-MS/MS analysis. This approach allowed us to identify proteins that co-purify with the TOM complex under native conditions. Our primary aim was to uncover human-specific interactors not previously described in yeast, with a particular focus on factors potentially involved in mitochondrial protein import regulation and quality control. The dataset provides a resource for understanding conserved and divergent aspects of TOM complex composition and function in human cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell

SUBMITTER: Remigiusz Serwa

LAB HEAD: Agnieszka Chacinska

PROVIDER: PXD064520 | Pride | 2026-03-17

REPOSITORIES: Pride

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			Action	DRS
	MB1_EV_1.raw	Raw
	MB_1WT_EV.raw	Raw
	MB_1WT_Flag.raw	Raw
	MB_2WT_EV.raw	Raw
	MB_2WT_Flag.raw	Raw

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