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Control of flagellar gene expression by the receptor-like regulator in pathogenic Escherichia coli

ABSTRACT: Chemoreceptors enable bacteria to modulate their swimming behavior in accordance with the perceived environmental cues. Bacteria exhibit large diversity of stimuli sensed by their chemoreceptors, whereas the output of receptors to the chemotaxis signaling pathway that controls flagellar motor is typically highly conserved. Here, we characterize a unique chemoreceptor-like protein, Tls, which is found in the B2 phylogroup of Escherichia coli that includes many extraintestinal pathogenic (ExPEC) strains. Instead of mediating chemotactic signaling, Tls controls motility by repressing the expression of flagellar genes, and thus cell motility, apparently by sequestering the transcriptional master activator of flagellar genes, FlhDC. Furthermore, we observe that the subcellular localization of Tls, the sequestration of FlhDC, and the repression of flagellar genes and motility are all abolished during growth on porous medium, indicating that this regulation may be mechanosensitive. Deletion of tls in a uropathogenic E. coli leads to reduced attachment to the urinary tract cells, but an increased migration to and/or proliferation in the murine gut, a pathogen reservoir niche, thus implicating Tls in the regulation of motility during infection.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Escherichia Coli

SUBMITTER: Timo Glatter

LAB HEAD: Timo Glatter

PROVIDER: PXD064677 | Pride | 2025-06-13

REPOSITORIES: Pride

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