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Global Matrisome changes in the obese lung are linked to fibroblastic stroma and premature aging

ABSTRACT: Obesity is associated with an increased risk of lung disorders, yet its effects on lung tissue structure and the microenvironment remain incompletely understood. Using a multiomics strategy combining tissue proteomics, lipidomics, solubility profiling, single-cell transcriptomics, and functional assays, we investigated how obesity affects lung composition and fibroblast behavior. Our data indicate that obesity induces remodeling of the extracellular matrix, including changes in elastic fiber-associated proteins, and is accompanied by reduced levels of protease inhibitors in lung tissue and BALF. Stromal fibroblasts accumulated lipids and exhibited increased contractility, matrisome alterations, and transcriptional hallmarks of aging. Comparison of obese and aging lungs revealed overlapping solubility changes in structural matrix proteins. These findings suggest that obesity may induce premature aging of fibroblasts and consequent remodeling of the lung microenvironment, with potential implications for tissue compliance and repair responses.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bronchoalveolar Lavage

SUBMITTER: Veronika Lukacs-Kornek

LAB HEAD: Veronika Lukacs-Kornek

PROVIDER: PXD064724 | Pride | 2025-08-21

REPOSITORIES: Pride

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			Action	DRS
	20250728_083620_BAL-Proteome.sne	Other
	241011_P-470-VL_060.raw	Raw
	241011_P-470-VL_061.raw	Raw
	241011_P-470-VL_062.raw	Raw
	241011_P-470-VL_063.raw	Raw

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Global Matrisome changes in the obese lung are linked to fibroblastic stroma and premature aging

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