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Proteomics of functional and dysfunctional hybridomas

ABSTRACT: Proteomics sequencing was utilized to investigate the key pathways and genes regulating hybridoma homeostasis and antibody production. Two phenotypes, including functional and dysfunctional hybridomas exhibiting and lacking binding specificity against chlorothalonil (a representative fungicide), were screened via limiting dilution and used for sequencing.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Rubing Zou

LAB HEAD: Yirong Guo

PROVIDER: PXD064735 | Pride | 2026-04-06

REPOSITORIES: Pride

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			Action	DRS
	Gf09LXY_D4_1_4_D1_1_4390.d.rar	Other
	Gf09LXY_D4_2_5_E1_1_4391.d.rar	Other
	Gf09LXY_D4_3_6_F1_1_4392.d.rar	Other
	Gf09LXY_E2_1_1_A1_1_4387.d.rar	Other
	Gf09LXY_E2_2_2_B1_1_4388.d.rar	Other

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Publications

Quantitative Proteomics and CRISPR/Cas9 Editing Reveal UPR-Mediated Control of Immunoglobulin Homeostasis in Hybridomas.

Zou Rubing R Lu Xinying X Liu Ying Y Yang Peiyu P Sun Shuo S Liu Yihua Y Mo Yinyuan Y Zhu Guonian G Lee Jae Seong JS Guo Yirong Y

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20260120 17

Despite their tremendous economic value, monoclonal antibodies are often compromised by the loss of immunoglobulin (Ig) chains, which disrupts antibody homeostasis and quality control. Through subclone screening and characterization, we identified that the loss of Ig production impaired the recognition ability of hapten-specific hybridomas. Proteomic analysis further highlighted the critical role of the unfolded protein response (UPR) pathway in regulating aberrant Ig chain production. Using CRI ...[more]

PMID: 41560288

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Quantitative profiling of proteome extracted from zebrafish embryos exposed to different concentrations of the fungicide difenoconazole against untreated control groups.

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