Dataset Information

COMPARATIVE PROTEOMICS ANALYSIS OF THE IRIDOCORNEAL REGION OF DBA/2J AND DBA/2J-Gpnmb+/SjJ MICE

ABSTRACT: In this study, we performed a comparative proteomics analysis of the iridocorneal region in DBA/2J and Gpnmb+ mice to gain deeper insight into the molecular mechanisms underlying IOP elevation following iris pigment dispersion/

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Eye Trabecular Meshwork, Anterior Chamber Of The Eye

DISEASE(S): Ocular Hypertension,Pigment Dispersion Syndrome

SUBMITTER: Paloma Liton

LAB HEAD: Paloma Liton,

PROVIDER: PXD064786 | Pride | 2026-02-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1d_D2_WT_ID125210_01_SPD30_OA10222_10809_013025.raw	Raw
	20250331_150028_10809_realign_paloma_033125.sne	Other
	525_gpnmb_ID125213_01_SPD30_OA10222_10809_013025.raw	Raw
	526_gpnmb_ID125214_01_SPD30_OA10222_10809_013025.raw	Raw
	5c_D2_WT_ID125211_01_SPD30_OA10222_10809_013025.raw	Raw

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Publications

Comparative Multi-Omics Analysis of the Iridocorneal Angle Identifies an Immune-Fibrotic Profile in the DBA/2J Glaucoma Mouse Model.

Shim Myoung Sup MS Grimsrud Aleks A Desikan Vaibhav V Sung Mi Sun MS Liton Paloma B PB

Molecular & cellular proteomics : MCP 20251222 2

We present the first integrated transcriptomic and proteomic profiling of the iridocorneal region in the spontaneous murine glaucoma model DBA/2J and DBA/2J-Gpnmb<sup>+</sup>/Sj controls to define molecular changes associated with ocular hypertension and glaucoma. Using RNA sequencing and label-free quantitative proteomics, we identified over 20,000 transcripts and 8500 proteins, creating a comprehensive molecular atlas of glaucoma-related alterations in DBA/2J mice. Principal component and diff ...[more]

PMID: 41443436

Similar Datasets

Project description:Purpose: The DBA/2J mouse is a model for secondary angle-closure glaucoma due to iris atrophy and pigment dispersion, which ultimately leads to increased intraocular pressure (IOP). We sought to correlate changes in retinal gene expression with glaucoma-like pathology by performing microarray analysis of retinal RNA from DBA/2J mice at 3 months before disease onset, and at 8 months, after IOP elevation. Methods: IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months. Results: There were changes in expression of 68 genes, with 32 genes increasing and 36 genes decreasing at 8 months versus 3 months. Upregulated genes were associated with immune response, glial activation, signaling and gene expression, while down-regulated genes included multiple crystallin genes. Significant changes in 9 upregulated genes and 2 downregulated genes were confirmed by quantitative RT-PCR, with some showing changes in expression by 5 months. Conclusions: DBA/2J retina shows evidence for glial activation and an immune-related response following IOP elevation, similar to what has been reported following acute elevation of IOP in other models. IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months.