Proteomics

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Development of Cellular Energy Metabolism During Differentiation of Human iPSCs Into Cortical Neurons

ABSTRACT: We performed label-free quantitative proteomic profiling to characterize metabolic remodeling during early differentiation of human iPSCs into excitatory cortical neurons induced by NGN2 overexpression. Proteomic analysis was conducted at three time-points (iPSCs, day 7, and day 14 post-induction), focusing on neuronal, mitochondrial, and biosynthetic pathways. Our findings identify the first week of neuronal differentiation as a critical period characterized by coordinated proteomic and metabolic reprogramming, including the suppression of pluripotency markers, acquisition of neuronal identity, and reduced proliferation. Mitochondrial biogenesis is initiated during this phase, accompanied by the upregulation of oxidative phosphorylation and fatty acid oxidation. These data establish a proteomic baseline for cortical neuron differentiation and provide a reference platform for the interpretation of metabolic alterations in patient-derived models.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Marek Vrbacky  

LAB HEAD: Jakub Otahal

PROVIDER: PXD064787 | Pride | 2025-11-24

REPOSITORIES: Pride

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PLK218_OE480_240308_FGU33_SDD14A_3uL_DIA-FAIMS_120M_90G.raw Raw
PLK218_OE480_240308_FGU33_SDD14B_2-8uL_DIA-FAIMS_120M_90G.raw Raw
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Development of Cellular Energy Metabolism During Differentiation of Human iPSCs into Cortical Neurons.

Danačíková Šárka Š   Pecina Petr P   Pecinová Alena A   Svoboda Jan J   Vondrášek David D   Basello Davide Alessandro DA   Čajka Tomáš T   Hadraba Daniel D   Mráček Tomáš T   Kořínek Vladimír V   Otáhal Jakub J  

Molecular neurobiology 20251113 1

Neuronal differentiation requires extensive metabolic remodeling to support increased energetic and biosynthetic demands. Here, we present an integrated multi-omics and functional characterization of metabolic transitions during early differentiation of human induced pluripotent stem cells (iPSCs) into excitatory cortical neurons using doxycycline-inducible overexpression of neurogenin-2 (NGN2). We analyzed parental iPSCs and induced neurons (iNs) at days 7 and 14 of differentiation, integrating  ...[more]

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