Dataset Information

Proteomic signatures of the chemotherapy-induced damage in the human ovary

ABSTRACT: Although the mechanisms underlying chemotherapy-induced damage to germ cells are being increasingly deciphered, its impact on the ovarian microenvironment remains largely unexplored. The ovarian stroma is equally exposed to chemotherapy, and since its cells and components are in active communication with follicles, any microenvironmental changes induced by chemotherapy might affect follicles as well. The present project aimed to characterise the alterations of the human ovarian microenvironment and to comprehensively elucidate the mechanisms of ovarian injury in response to chemotherapy. Human ovarian fragments from women previously exposed or not to first-line chemotherapy were analysed using mass spectrometry and bioinformatics. A total of 5,209 proteins were detected in both chemotherapy-exposed and control ovaries. Comparative proteomic analysis revealed 237 differentially expressed proteins (4.5%) post-treatment, among which 162 were upregulated and 75 were downregulated in ovarian cells from chemotherapy-treated patients compared to controls. Biological pathways related to immune response, hypoxia and apoptosis were upregulated following chemotherapy exposure, while those involved in cell cycle and DNA repair were downregulated. Markers of the ECM network were also dysregulated. These findings provide evidence that chemotherapy significantly alters the ovarian stroma, emphasizing the need to further investigate the long-term consequences for fertility and reproductive lifespan after transplantation. They also suggest target proteins which may drive these chemotherapy-associated ovarian damage for future pharmacoprotection investigations.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Ovary

SUBMITTER: Xavier Bisteau

LAB HEAD: Isabelle Demeestere

PROVIDER: PXD064815 | Pride | 2025-11-07

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20240216_MS025_E1_S1-10_DIAnn_report.pg_matrix.tsv	Tabular
	20240216_MS025_E1_S1-10_DIAnn_report.stats.tsv	Tabular
	20240216_MS025_E1_S1-10_DIAnn_report.tsv	Tabular
	MS025_E1_S10_swath_6u_inj1.mzML	Mzml
	MS025_E1_S10_swath_6u_inj1.mzML.quant	Mzml

Items per page:

1 - 5 of 59

Similar Datasets

Project description:Ovarian follicular granulosa cells surround and nurture oocytes, and produce sex steroid hormones. It is believed that during development the ovarian surface epithelial cells invaginate into the ovary and develop into granulosa cells when associating with oogonia to form follicles. Using bovine fetal ovaries (n = 53) we identified a novel cell type, termed GREL for Gonadal Ridge Epithelial-Like. Using 25 markers for GREL and other cells we conducted immunohistochemistry and electron microscopy and chronologically tracked all somatic cell types during development. Before 70 days of gestation the gonadal ridge/ovarian primordium is formed by proliferation of GREL cells at the surface epithelium of the mesonephros. Primordial germ cells (PGCs) migrate into the ovarian primordium. After 70 days, stroma from the underlying mesonephros begins to penetrate the primordium, partitioning the developing ovary into irregularly-shaped ovigerous cords composed of GREL cells and PGCs/oogonia. Importantly we identified that the cords are separated from the stroma by a basal lamina. Around 130 days of gestation as the stroma expands laterally below the GREL cells on the surface thus establishing a sub-epithelial basal lamina and an epithelial-stromal interface, and it is at this stage that a mature surface epithelium develops from the GREL cells. The stroma continues to partition the ovigerous cords into smaller groups of cells eventually forming follicles containing an oogonium/oocyte surrounded by GREL cells, which become granulosa cells. Thus in contrast to the prevailing theory, the ovarian surface epithelial cells do not invaginate into the ovary to form the granulosa cells of follicles. Microarray analysis of gene expression was conducted on different cell types cultured from fetal ovaries to identify possible markers of somatic cells during development. Two different cell types: Gonadal Ridge Epithelial-Like cells (n=2, 130 days gestation) and adult fibroblasts (n=1) were cultured from digested bovine fetal ovaries and gene expression compared with each other by Bovine Genome Affy array analysis in Partek Genomics Suite software, to identify possible markers of somatic cells during development.

Dataset Information

Proteomic signatures of the chemotherapy-induced damage in the human ovary

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets