Dataset Information

Lysine acetylomic profiling of hybrid rice HY73 in response to drought

ABSTRACT: This study investigates the molecular mechanisms underlying drought-resistant heterosis (DRH) in the elite hybrid rice HY73. Utilizing a quantitative lysine acetylome profiling approach on flag leaves of HY73 under well-watered (WW) and drought-stressed (DS) conditions, we aimed to unravel the role of protein lysine acetylation (Kac) in carbon metabolic reprogramming during drought adaptation. Critically, we identified phosphoglucomutase (OsPGM3) as a key metabolic switch whose K155 acetylation (K155ac) is dynamically downregulated under DS. This regulation orchestrates a strategic carbon reallocation, optimizing glucose-6-phosphate (G6P) flux towards lipid biosynthesis for stress resilience (MOS-AcCoA-FA/PR pathway) while maintaining efficient glucose-1-phosphate (G1P)-mediated sucrose export for yield potential. This dataset provides valuable insights into the sophisticated post-translational regulatory network that underpins superior DRH and yield stability in hybrid rice under water scarcity.

INSTRUMENT(S):

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Leaf

SUBMITTER: Wenbo Cheng

LAB HEAD: Wei Ding

PROVIDER: PXD064916 | Pride | 2026-06-28

REPOSITORIES: Pride

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Publications

Integrated multi-omics analysis reveals carbon metabolic reprogramming as a mechanism of drought resistance in hybrid rice.

Ding Wei W Bi Junguo J Chen Shoujun S Wu Zhen Z Liao Zhigang Z Huang Lin L Luo Lijun L Chen Liang L Zhang Xumin X

Plant communications 20260526

Drought severely limits global rice production, and the exploitation of heterosis offers a promising route for improving drought resistance. However, the molecular mechanisms that underlie drought resistance in hybrid rice remain largely unknown. Here, we performed integrated multi-omics analyses of flag leaves from the elite hybrid rice Hanyou73 (HY73) and its parental lines, Hanhui3 (HH3) and Huhan7B (HH7B), during grain filling under drought. We discovered that non-additive effect (NAE)-drive ...[more]

PMID: 42198838

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Project description:Cotton (Gossypium hirsutum L.) is one of the most important cash crops worldwide. In semi-arid/arid regions, drought stress causes growth limitation and decrease of yield. Of all the organs of a plant, fine root is the central part consisting of the root system to contribute to plant water and nutrient taken up. However, the research on the molecular mechanism underlying fine root response to soil drought has not been well understood in cotton. To better characterize the proteomic changes of cotton fine roots under drought stress, 70±5% and 40±5% soil relative water content were designed as control (CK) and drought stress (DS) groups, respectively. Tandem mass tags (TMT) technology was used to determine the proteome profiles in fine roots. The proteomic differences between CK and DS were pairwise compared at 0, 30, and 45 days after drought stress (DAD). A total of 11,628 proteins were identified, of which 10,344 proteins contained quantitative information. According to the morphological, physiological, and biochemical characteristics, 30 and 45 DAD were selected as critical stages for further analysis. Results showed that 118 differentially expressed proteins (DEPs) were up-regulated and 105 down-regulated in DS 30 versus CK 30; 662 DEPs were up-regulated, and 611 were down-regulated in DS 45 versus CK 45. The DEP functions were determined for their classified pathways, mainly associated with carbohydrate metabolism, energy metabolism, fatty acid metabolism, amino acid metabolism, and secondary metabolite biosynthesis. DEPs related to phytohormone and stress/defense response were also identified. To verify the accuracy of the TMT results, 20 DEPs were randomly selected for parallel reaction monitoring (PRM) verification. And results showed that the quantitative results of TMT are consistent with those of PRM, which proved that the TMT results of this study are reliable. In this article we describe changes in the protein profiles occurring in response to drought stress in cotton fine roots. Proteomic analyses of plant responses to stressors could lead to the introduction of cotton cultivars with high resistance to drought stress. Such plants would be valuable for high yielding under drought as well as other unfavorable environmental conditions.

			Action	DRS
	AcetylK_DDA_1.raw	Raw
	AcetylK_DDA_2.raw	Raw
	AcetylK_DDA_3.raw	Raw
	AcetylK_HY73_DIA_DS-1.raw	Raw
	AcetylK_HY73_DIA_DS-2.raw	Raw