Dataset Information

Multi-omic responses of mouse liver and skeletal muscle to chronic alcohol drinking

ABSTRACT: The aim of this study was to define and compare the molecular blueprints of chronic alcohol use in the liver and skeletal muscle using an established pre-clinical mouse model.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle, Liver

SUBMITTER: Craig Willis

LAB HEAD: Craig Willis

PROVIDER: PXD064997 | Pride | 2025-12-11

REPOSITORIES: Pride

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			Action	DRS
	202207011_SJMS_Eclipse_FAIMS_UOhio_1of15uL_DIA_Lib_frac19.raw	Raw
	202207011_SJMS_Eclipse_FAIMS_UOhio_1of15uL_DIA_Lib_frac20.raw	Raw
	202207011_SJMS_Eclipse_FAIMS_UOhio_1of15uL_DIA_Lib_frac21.raw	Raw
	202207011_SJMS_Eclipse_FAIMS_UOhio_1of15uL_DIA_Lib_frac22.raw	Raw
	202207011_SJMS_Eclipse_FAIMS_UOhio_1of15uL_DIA_Lib_frac23.raw	Raw

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Project description:Abstract: Candida parapsilosis and Candida albicans are human fungal pathogens that belong to the CUG clade in the Saccharomycotina. In contrast to C. albicans, relatively little is known about the virulence properties of C. parapsilosis, a pathogen particularly associated with infections of premature neonates. We describe here the construction of >200 C. parapsilosis strains carrying double allele deletions of transcription factors, protein kinases and species-specific genes. Two independent deletions were constructed for each target gene. Growth in > 40 conditions was tested, including carbon source, temperature, and the presence of antifungal drugs. The phenotypes were compared to C. albicans strains with deletions of orthologous transcription factors. We found that many phenotypes are shared between the two species, such as the role of Upc2 as a regulator of azole resistance. Others are unique. For example, Cph2 plays a role in the hypoxic response in C. parapsilosis and not in C. albicans. We found extensive divergence between the biofilm regulators of the two species. We identified 7 transcription factors and one protein kinase that are required for biofilm development in C. parapsilosis. Only three (Efg1, Bcr1, and Ace2) have similar effects on C. albicans biofilms, whereas Cph2, Czf1, Gzf3 and Ume6 have major roles in C. parapsilosis only. In addition, two transcription factors (Brg1 and Tec1) with well-characterized roles in biofilm formation in C. albicans do not have the same function in C. parapsilosis. We also compared the transcription profile of C. parapsilosis and C. albicans biofilms. Our analysis suggests the processes shared between the two species are predominantly metabolic. C. parapsilosis mRNA profiles of wild type (WT) at 37 degree celcius in planktonic growth conditions and ace2-/-, cph2-/-, efg1-/-, czf1-/-, ume6-/-, bcr1-/- and WT in biofilm conditions were generated by deep sequencing, in triplicate, using Illumina HiSeq2000.

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Multi-omic responses of mouse liver and skeletal muscle to chronic alcohol drinking

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