Bioresponsive pseudoGlucosinolates (psGSLs) release Isothiocyanates (ITCs) in the Presence of Nitroreductases

ABSTRACT: Glucosinolates (GSLs) are secondary metabolites produced as part of an herbivore defence system in plants of the order Brassicales. GSLs release isothiocyanates (ITCs) upon activation by the myrosinase. Beyond their herbivore feeding deterrent properties, these ITCs have multiple interesting bioactivities. However, their release is limited by the presence of myrosinase. Here, we report the concept of pseudoglucosinolates (psGSLs) hijacking the natural release mechanism of GSLs for the release of ITCs and adapting it to nitroreductase as the triggering enzymes. We provide proof-of-concept for nitroreductase-responsive psGSLs and demonstrate their potential for peptide labelling, ITC-prodrug approaches and for in vivo applications using the nematode of C. elegans

INSTRUMENT(S):

ORGANISM(S): Synthetic Construct (code 12)

SUBMITTER: Liisa Arike  

LAB HEAD: Philipp Klahn

PROVIDER: PXD065044 | Pride | 2026-09-07

REPOSITORIES: Pride