Identification of the ubiquitin linkages generated by ANKIB1

ABSTRACT: moTAP-ANKIB1 was purified from transduced HeLa by using M2 beads followed by streptavidin beads. Once purified, in vitro ubiquitin assay was performed in cell free assay during 1h at 37°C. Purified ANKIB1 was incubated with or without UBE1, UBE2D3, ATP regeneration solution and ubiquitin molecules.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Proteomics Facility  

LAB HEAD: Henning Walczak

PROVIDER: PXD065049 | Pride | 2026-03-03

REPOSITORIES: Pride