Dataset Information

Serum H-ferritin to Ferritin ratio as a biomarker of HLH in critically ill patients with hyperferritinemia

ABSTRACT: Ferritin is a sensitive screening parameter to diagnose hemophagocytic lymphohistiocytosis in critically ill patients but lacks specificity. Ferritin is composed of 24 subunits with 2 types of peptide chains: light (FeL) or heavy (FeH). The proportion of H subunit is suggested to increase during hyperinflammation but is not routinely evaluated by the commercial immunoassay. To improve the specificity of the assay for HLH, we performed proteomic analysis to quantify both FeL and FeH in the sera of patients with hyperferritinemia suspected of HLH or with sepsis admitted in intensive care units. Starting from an untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of 6 human serum samples with hyperferritinemia (>5000ug/L), we developed a targeted LC-MS/MS method based on multiple reaction monitoring (MRM) to quantify FeH in human serum and evaluate the diagnostic performance of the L and H subunit (FeL and FeH) in our cohort of critically patients with hyperferritinemia.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sara Dufour

LAB HEAD: Debaugnies France

PROVIDER: PXD065102 | Pride | 2026-03-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	B101810_Bp_uPac18_trap2_PRC-5683_Borde_Debaugnies_shotgun_DDA_1.raw	Raw
	B101812_Bp_uPac18_trap2_PRC-5683_Borde_Debaugnies_shotgun_DDA_2.raw	Raw
	B101814_Bp_uPac18_trap2_PRC-5683_Borde_Debaugnies_shotgun_DDA_3.raw	Raw
	B101816_Bp_uPac18_trap2_PRC-5683_Borde_Debaugnies_shotgun_DDA_4.raw	Raw
	B101818_Bp_uPac18_trap2_PRC-5683_Borde_Debaugnies_shotgun_DDA_5.raw	Raw

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Publications

Serum H-Ferritin-To-Ferritin Ratio as a Biomarker of Hemophagocytic Lymphohistiocytosis in Critically Ill Patients With Hyperferritinemia.

Debaugnies France F Goetzinger Frank F Wolff Fleur F Impens Francis F Dufour Sara S Van Haver Delphi D Gottignies Philippe P La Schiazza Raphaël R Mahadeb Bhavna B Meuleman Nathalie N Nagant Carole C Rozen Laurence L Borde Patricia P Corazza Francis F

American journal of hematology 20260106 3

PMID: 41492914

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